RECK基因和基质金属蛋白酶2在食管鳞癌中的表达及其临床病理意义

摘要

Objective To investigate the role and clinicopathological significance of RECK and MMP-2 expressions in esophageal squamous cell carcinoma(ESCC).Methods We collected 57samples of ESCC tissue and 57 samples of corresponding normal esophageal epithelium tissues in Henan Cancer Hospital.The expression of RECK and MMP-2 was detected with tissue array and immunohistochemistry.Chi-square test and Spearman corrilation analysis were used to analyze the results.Results The positive rate of MMP-2 protein expression in cancer tissues was 42.1%(24/57),higher than in normal esophageal epithelia 19.3%(11/57),with a significant difference(χ2=6.968,P＜0.05).For ESCC,a difference was not found in MMP-2 expression among patients with different degree of differentiation and different depth of invasion.The expression levels of RECK in ESCC tissues and normal esophageal epithelia were 36.8%(21/57)and 61.4%(35/57),respectively,with a significant difference(χ2=6.879,P＜0.05).For ESCC,the expression level of RECK was not related to the degree of differentiation,depth of invasion and lymph node metastasis(P＞0.05).For ESCC patients,the expression rates of RECK and MMP-2 were not correlated with age or gender.Conclusion The abnormal expression level of the RECK and MMP-2 protein may play an important role in carcinogenesis of esophageal squamous carcinoma.Decreased expression of RECK and increased expression of MMP-2 may be correlated with the esophageal squamous cell carcinoma development,but there is no connection observed between the expression of MMP-2 and RECK.%目的 探讨RECK基因和基质金属蛋白酶2(MMP-2)在食管鳞癌发生、发展中的作用.方法 收集河南省肿瘤医院食管鳞癌标本57例,同时取患者相应的癌旁正常食管黏膜组织作为对照.应用组织芯片法与免疫组织化学法,检测每例食管鳞痛标本和正常食管黏膜组织中RECK和MMP-2的表达情况.结果 RECK在食管鳞癌组织中的表达率为36.8%(21/57),在正常食管黏膜组织中的表达率为61.4%(35/57),两组间差异有统计学意义(χ2=6.879,P＜0.05);RECK的表达与患者的年龄、性别、肿瘤组织学分级、淋巴结转移无关(均P＞0.05),但与临床病理分期有关(P＜0.05).MMP-2在食管鳞癌组织中的阳性表达率为42.1%(24/57),在正常食管黏膜组织中的阳性表达率为19.3%(11/57),两组间差异有统计学意义(χ2=6.968,P＜0.05);MMP-2的表达与患者的年龄、性别、肿瘤组织学分级无关(均P＞0.05),但与肿瘤临床病理分期和肿瘤淋巴结转移有关(P＜0.05).此外,RECK和MMP-2在食管鳞癌中阳性表达差异无统计学意义(P＞0.05).结论 RECK和MMP-2的异常表达在食管鳞癌的发生中有重要作用,RECK的低表达和MMP-2的高表达与食管鳞癌临床病理进展有关.