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蓖麻枯萎病抗性的QTL定位分析

     

摘要

In this study, the QTLs conferring the resistance to Fusarium wilt were mapped with the F2 populations derived from YC2 × YF1 resistant and susceptible combination under 2 environments in castor. Genetic analysis presented a continuous skewness distribution in populations, tending to resistant parent. 5 QTLs were identified in 2014, explained 18. 21% of phenotype variation, with a single locus contribution rate of 0. 05%-12. 32%.Dynamic mapping was performed at 5 stages including 20 d, 30 d, 40 d after seedling, squaring stage and milk ripening period in 2015, and 4, 12, 13, 4 and 1 QTLs were detected respectively, with a single locus contribution rate of 1. 64%-21. 91%. 1, 3 and 6 QTLs were identified simultaneously at 4, 3 and 2 stages respectively. The detected QTLs at these 5 stages were mainly concentrated on linkage group 3, 4 and 8. One QTL located on linkage group 8 was detected simultaneously in both environments at the same stage. The above results could facilitate the molecular marker assisted selection of Fusarium wilt resistance in castor.%本研究用YC2×YF1抗、感组合的F2群体在两个环境中对蓖麻枯萎病抗性进行了QTL定位.遗传分析表明,群体中枯萎病抗性呈连续性偏态分布,后代表型偏抗性亲本.2014年检测到5个QTL,解释了总变异的18.21%,单位点贡献率为0.05%~12.32%.2015年分别在出苗后20d、30d、40d、现蕾期和乳熟期进行了动态定位,分别检测出4、12、13、4和1个QTL,单位点贡献率为1.64%~21.91%,5个发育时期检出的QTL主要集中在第3、4和8连锁群上.有1个QTL在4个时期、3个QTL在3个时期、6个QTL在2个时期被重复检测到.在第8连锁群上一个QTL在两个环境同一时期被重复检测到.以上结果可为蓖麻抗枯萎病分子标记辅助选择提供参考.

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