富含硫氨基酸转基因大豆对根际土壤微生物群落结构的影响

摘要

选用两组转基因品质改良大豆(富含硫氨基酸),利用磷脂脂肪酸( PLFA)法分析了在大田种植条件下,两组转基因大豆对土壤微生物群落结构的影响.结果表明,与非转基因大豆相比,转基因品系根际土壤微生物群落的结构发生明显的变化.在两组转基因品系中PLFA组分均发生变化,A组中检测到15种特异性的PLFA,其中占主导地位的分别是是革兰氏阳性菌(GP)的特征脂肪酸16:0、10Me18:0和好氧细菌特征脂肪酸18:1w7c;B组中检测到13种特异性的PLFA,其中占主导地位的是表征GP的脂肪酸15:0、16:0和非特征脂肪酸14Me16:0.在A组的OE -8品系及B组的17 -4和57品系中,表征细菌的PLFA含量和总PLFA含量与非转基因品系相比有显著上升.结果表明种植转基因大豆影响根际土壤微生物群落结构.%Effects of transgenic soybeans on microbial population structure of rhizosphere soil were investigated under field conditions based on phospholipid fatty acid (PLFA) method. Two groups of transgenic soybeans with sulfur - rich amino acids were used. Results showed that soil microbial population in rhizosphere of transgenic lines changed significantly in both group A and B. 15 specific PLFAs were identified in group A, and 13 in group B. Correlation analysis found that microbial characterization of group A and the total concentration of PLFA' s of the transgenic line OE -8 were significantly higher than non -transgenic line, and so were 17-4 line and 57 line in group B. The dominant fifteen PLFA mown plots were 16: 0, 10Mel8: 0 and 18: 1ω7c in group A, and dominantrnthirteen PLFA were 15: 0, 16: 0 and 14Mel6: 0 in group B. The results suggested that the microbial population structure changed after planting transgenic soybeans.