多环境条件下大豆蛋白质含量稳定性Q TL分析

摘要

为定位大豆蛋白质含量稳定性QTL，从而为培育高蛋白大豆品种提供依据，本研究利用源自美国大豆Charleston和中国品种东农594杂交获得的147个株系组成的重组自交系群体，利用三种生态环境下三年数据估算的Shukla稳定性方差对大豆蛋白含量进行了遗传和QTL分析。结果表明，利用复合区间作图法（CIM）检测大豆蛋白稳定性QTL得到2个QTL，分别为qPRO1-1和qPRO17-1，位于连锁群A1和L上，贡献率分别为4．70％和5．73％，共解释10．43％的表型变异。利用混合区间作图法（MIM）检测到2对上位性QTL，互作染色体为A1×G和A1×A2，上位效应分别为0．19**和-0．22，贡献率为12．82％和17．42％，共解释30．24％表型变异。本实验分析多个环境下的数据，考虑到了QTL与环境的互作效应，在三种环境条件下分析QTL，检测到了在不同环境下可以稳定出现的QTL位点。控制大豆蛋白含量的QTL位点，都表现出明显的上位性效应和GE互作效应。其中稳定性较好的QTL和公共图谱上定位的调控大豆蛋白质含量的QTL prot 1-7、cq oil003、oil8-1、prot 17-5、prot 2-1及prot 12-1等在区间上一致。%The objective of this study is to identify the consensus QTLs underlying protein content of soybean, and provide a foundation for molecular assistant breeding.The population of 147 recombination inbred lines (RIL) derived from the cross of America cultivar Charleston and Chinese variety Dongnong 594,was used to analyze the genetic and QTL of protein content in 3 environments for 3 years.The stability QTLs of protein content was esti-mated using Shukla ANOVO method.2 QTLs,qPRO1 -1 and qPRO17 -1 ,located on linkage group A1 and L, could explain the variance of 4.70%and 5 .73%respectively.2 pairs of epistatic QTLs,located on linkage groups of A1 ×G and A1 ×A2,found by MIM model method analysis,their epistatic effect was 0.19** and -0.22, could explain 12.82% and 17.42% variance respectively.Multi-environmental data was analyzed in this study, which benefited the study of interaction between QTL and environment.In three different environments,the stabili ty QTL was identified.The QTL underlying protein content had significant epstatic and QE interaction.There were promising consistency between the stable QTL and the known QTL on soybean composite genetic map,including prot 1 -7,cq oil003,oil8-1,prot 17-5,prot 2-1 and prot 12-1.