多环境条件下大豆异黄酮主要组分的QTL定位

摘要

[目的]利用不同定位方法,对不同环境条件下大豆异黄酮主要组分进行QTL定位研究,为大豆异黄酮分子标记辅助育种提供理论依据.[方法]以异黄酮含量有显著差异的鲁黑豆2号(3 697.24 μg·g-1)和南汇早黑豆(1 816.67 μg·g-1)为亲本构建的F5∶7-8重组自交系为材料,分析RIL群体的SSR标记多态性,结合HPLC法鉴定异黄酮主要组分含量.[结果]绘制了一张包含161个多态性SSR标记,全长3 546.54 cM的大豆遗传连锁图谱.利用ICIMapping 3.2软件的ICIM、IM和SMA 3种定位方法,共定位到4种环境下与异黄酮主要组分相关的14个QTL.[结论]3个标记区间在多个环境和多种定位方法下均被检测到,分别是Sat_003-Satt306、Satt070-Satt122和Satt571-Satt270.%[Objective] The QTLs for major isoflavone components were identified among multiple environments by different QTL mapping methods in soybean seeds, in order to provide a theoretical basis for soybean isoflavone marker-assisted selection.rn[Method] In this study, a recombinant inbreed line population (RIL, F5:7-8) was developed by using the cross between the isoflavone contrasting cv. LHD2 with high-isoflavone concentration (3 697.24 ug-g"1) and cv. NHZ with low-isoflavone concentration (1 816.67 μg.g-1). The polymorphism of SSR markers among the RIL population was analyzed and the major isoflavone components were determined by HPLC. [Result] The results showed that a soybean linkage map with a total distance of 3 546.54 cM was constructed using 161 polymorphism SSR molecular markers in the RIL population. A total of 14 QTLs associated with the major isoflavone components were found by ICIM, IM, and SMA methods in the ICIMapping 3.2 software under the four environments.rn[Conclusion] The three QTLs flanked by the marker interval Sat_003-Satt306, Satt070-Sattl22 and Satt571-Satt270 were detected among multiple environments in more than two methods.