晶状体皮质诱导视网膜前新生血管膜形成的实验研究

摘要

目的 观察自体晶状体皮质与视网膜前新生血管膜形成的关系.方法 选择4～6周健康C57BL/6小鼠24只,随机分为实验组和对照组,每组各12只.实验组,采用微创方法穿破小鼠晶状体后囊膜,让其晶状体皮质缓慢释放入玻璃体腔内;对照组在不损伤晶状体的情况下,采用微创方法玻璃体腔内注射等量磷酸盐缓冲液(PBS).常规眼部检查和裂隙灯照相.在微创穿破晶状体后囊膜手术后第3、7、14、28 d,分别取眼球进行组织病理和免疫组织化学检查.结果 手术后1～3 d,12只实验眼中,11只眼晶状体相继发生不同程度的白色混浊;另1只眼到实验结束时,未发生晶状体混浊.手术后3 d,玻璃体腔内可见到游离的晶状体皮质碎片,向视网膜靠近,部分接触皮质的视网膜内界膜粗糙,连续性中断;7～14 d视网膜内邻近内界膜的毛细血管扩张、迁移呈泡状突破内界膜,形成视网膜前新生血管膜;28 d,视网膜前邻近的玻璃体腔内能见到厚壁的血管样结构.对照组均未见晶状体混浊和上述病理改变.结论 自体晶状体皮质能诱发视网膜前新生血管膜的形成.%Objective To investigate auto-cortex of crystalline lens-induced neovascular epiretinal membrane(NVERM)by micro-injuring posterior capsule of crystalline lens. Methods twenty four C57BL/6 mouse between 4-6 weeks were selected,and divided into two groups randomly:auto-cortex of crystalline group and the control group.The auto-cortex of crystalline group was treated by penetrating the posterior capsule of lens and washing out the lens cortex into the mouse vitreous using PBS(phosphate buffered solution),while the control group were injected PBS into vitreous merely.Clinical changes were followed by slit-lamp examination and photograph.The eye balls were enucleated at the day of 3,7,14and 28 after operation.Both HE and immunohistochemistry were used to detect the pathological changes.Results postoperative one to three days,11 of 12 mouse in auto-cortex of crystalline group,lens appear tO alba turbid at different levels one after another,and then develop into high-density chinaware white.Postoperative(po)three days,HE staining shows cortex of lens debris transmigrated in vitreous cavity,and some of which approached to internal limiting membrane and lead it to rough and discontinue;Po 7-14 days,the capillary in retina expanded,migrated and broke thought internal limiting membrane which got to the pro-retina and became the new vessels.And typical NVERM were observed.Po28 days,some vasculars-like structure formed in vitreous cavity.None of mouse in control group developed NVERM. Conclusion Auto-cortex of crystalline lens can induced neovascular epiretinal membrane in C57BL/6 mouse.