程序性死亡因子1及其配体在糖尿病视网膜病变患者外周血单个核细胞中的表达

摘要

目的 观察程序性死亡因子1(PD1)及其配体PD-L1和PD-L2在糖尿病视网膜病变(DR)患者外周血单个核细胞(PBMCs)中的表达.方法 临床检查确诊为DR的40例患者(DR组)以及同期行体检的健康志愿者20名(对照组)纳入研究.DR组40例患者中,非增生型DR(NPDR)20例,增生型DR(PDR) 20例.抽取两组受检者晨起空腹静脉血2 ml,采用荧光定量聚合酶链式反应(PCR)检测PD-1、PD-L1、PD-L2 mRNA的表达水平.对比分析DR、对照组之间,PDR与NPDR组之间各指标表达水平的差异.结果 荧光定量PCR检测发现,DR组患者PBMCs中PD-1、PD-L1 mRNA相对表达量显著低于对照组,差异有统计学意义(t=-2.060,-2.562；P=0.043,0.013)；PD-L2 mRNA相对表达量较对照组无明显变化,差异无统计学意义(t=-0.857,P=0.395).PDR组患者PBMCs中PD-1 mRNA相对表达量较NPDR组有降低趋势,PD-L1、PD-L2 mRNA相对表达量有升高趋势,但差异均无统计学意义(t=-1.335,0.987,0.131;P=0.190,0.334,0.897).结论 DR患者PBMCs中PD-1、PD-L1 mRNA相对表达较正常者低,PD-L2 mRNA相对表达较正常者无明显变化.%Objective To observe the expression of programed death-1 (PD-1) and its ligands including PD-L1 and PD-L2 in peripheral blood mono-nuclear cells (PBMCs) of patients with diabetic retinopathy (DR) patients.Methods Forty patients with DR (DR group) and 20 healthy controls (control group) were included in this study.There were 20 patients with non-proliferative DR (NPDR) and 20patients with proliferative DR (PDR).Peripheral Blood samples were obtained from two groups.Real time polymerase chain reaction (RT-PCR) was used to analyze PD-1,PD-L1,and PD-L2 mRNA expression in PBMCs.The clinical data was analyzed in DR group and controls,also in PDR group and NPDR group.Results The results of RT-PCR showed that the expression of PD-1 and PD-L1 mRNA in DR group were significantly lower than those in the control group (t=-2.060,-2.562; P=0.043,0.013).There was no significant difference in PD-L2 mRNA expression between DR and control group (t=-0.857,P=0.395).Compared with the NPDR group,the lower expression level of PD-1 mRNA and higher expression level of PD-L1 and PD-L2 mRNA in PDR group were observed,but the differences were not statistically significant (t=-1.335,0.987,0.131; P=0.190,0.334,0.897).Conclusion PD-1 and PD-L1 mRNA expression in PBMCs of DR patients is decreased compared with controls,but there are no differences in PD-L2 mRNA expression in them.