Objective To quantitatively detect circulating DNA levels in the plasma of patients withcervical lesion and to determine the value for diagnosis of cervical lesion and cervical cancer . Methods Preoperative blood samples were collected from 53 cases of low-grade lesions, 49 cases of high-grade lesions, 44 cases of cervical invasive cancer and 70 cases of healthy women. Plasma DNA was extracted by magnetic bead method (BILATEST DNA kit). The quantity of plasma DNA was determined by duplex real-time quantitative PCR. Results Median plasma DNA level of invasive cervical cancer patients was 61. 59 mg/L (32. 06 - 162. 16 mg/L) , which was significantly higher than that of healthy women [16. 35 mg/L(11. 98 -22.71 mg/L), P < 0.01]. Among invasive cervical cancer patients, median plasma DNA level of squamous carcinoma patients was slightly higher than that of adenocarcinoma (50. 43 versus 47. 31 mg/L,P>0. 05). Median plasma DNA level of stage I patients was lower than that of stage Ⅱ- Ⅲ patients (46. 02 versus 71. 35 mg/L, P <0. 05). Conclusion Quantitatively detecting plasma circulating DNA may be with some application prospect in the diagnosis of cervical diseases.%目的 对宫颈疾病患者血浆循环DNA进行定量检测,探讨该指标对宫颈疾病特别是官颈癌的诊断价值.方法 收集2009年5月至2010年12月在南京医科大学第一附属医院就诊的53例官颈低度病变[即宫颈上皮内瘤变(CIN)Ⅰ]、49例官颈高度病变(包含CINⅡ、CINⅢ)和44例原发性宫颈浸润癌患者的治疗前血浆,并选取同期体检的健康妇女70例作为对照.磁珠法提取血浆循环DNA,采用自行建立的含内参照的双重实时荧光定量PCR技术检测血浆循环DNA含量.结果 宫颈癌患者血浆循环DNA含量的中位数为61.59 mg/L,显著高于低度病变、高度病变、健康对照妇女(中位数分别为21.77、24.17、16.35 mg/L),分别比较,差异均有统计学意义(P<0.01).鳞癌患者血浆循环DNA含量(中位数为50.43 mg/L)高于腺癌(中位数为47.31 mg/L),两者比较,差异无统计学意义(P>0.05).Ⅰ期宫颈癌患者血浆循环DNA含量明显低于Ⅱ～Ⅲ期者(中佗数分别为46.02、71.35 mg/L),两者比较,差异有统计学意义(P<0.05).结论 血浆循环DNA在宫颈疾病的诊断及衡量疾病严重程度中具有一定的应用前景.
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