养血生发胶囊双波长融合HPLC特征图谱

摘要

Objective: To establish the HPLC characteristic chromatogram of Yangxueshengfa capsules at 240 nm and 310 nm by dual-wavelength fusing method. Methods: The HPLC analysis was performed on an Agilent ZORBAX Eclipse XDB-C18 column (250 mm x4.6 mm, 5 |xm). The column temperature was 30 ℃. Acetonitrile-0. 1% acetate acid aqueous solution was used as the mobile phase eluted gradiently. DAD detection was used to collect the signals at the two characteristic wavelengths. The HPLC characteristic chromatograms of ten batches of samples were determined. The characteristic chromatograms were comprehensively evaluated by ' the digitized evaluation system of the traditional Chinese medicine fingerprint with the super-information characteristics'. Results: Eighteen common peaks were identified in the HPLC characteristic chromatograms of ten batches of Yangxueshengfa capsules by using the peak of archen as referential peak. The similarity of the ten batches was over 0. 93. The methodology validation results were good. Conclusion: The dual-wave length fusing method can effectively control the quality of Yangxueshengfa capsules, overcome the limited information of mono-wavelength detection , and offer guarantee for revealing the characteristics of traditional Chinese medicine, which could provide evidence for the i-dentification and quality control of Yangxueshengfa capsules.%目的:建立养血生发胶囊240 nm和310 nm的双波长融合HPLC特征图谱.方法:样品色谱测定使用Agilent ZORBAX Eclipse XDB-C18色谱柱(250 mm× 4.6 mm,5μm),柱温30℃,以0.1％冰醋酸-乙腈为流动相进行梯度洗脱,同时采集2个特征吸收波长下的信号,记录10批样品的HPLC特征图谱;采用“中药色谱指纹图谱超信息特征数字化评价系统”对图谱进行融合分析.结果:以大黄素为参照物,10批样品所得融合特征图谱共标定18个共有峰,相似度均在0.93以上.方法的精密度、稳定性和重复性良好.结论:本试验建立的方法克服了单波长检测时信息量有限的缺点,能较全面、真实地揭示中药材的内在质量特征,可为养血生发胶囊的质量控制和评价提供依据.