Th22细胞和 IL-22在肝癌外周血的表达及与肿瘤标记物的相关性研究

摘要

目的探讨Th22和IL-22在肝细胞癌外周血的表达情况，并分析其与肿瘤标记物的相关性。方法抽取45例肝细胞癌患者和15名正常人的外周血标本，采用流式细胞术检查Th22细胞水平，ELISA法检测IL-22水平，分析Th22、IL-22水平与肿瘤标记物AFP、CA125、CA193、CA199、CEA的相关性。结果肝细胞癌患者Th22和IL-22水平均显著高于健康人群水平（ P＜0.05）。男性和女性患者Th22、IL-22水平差异无统计学意义（P＞0.05）。肝细胞癌患者外周血Th22细胞数量和血清IL-22水平存在正相关（r＝0.322，P＝0.033），而Th22数量和血清 IL-22水平与肿瘤标记物AFP、CA125、CA193、CA199、CEA 均无相关性（ P＞0.05）。结论尽管Th22和IL-22在肝癌外周血中高表达，但与血清肿瘤标记物无相关性，其在肝癌发病中的临床意义仍需临床大样本研究证实。%Objective To investigate the level of Th22 cells and IL-22 in peripheral blood of patients with hepatocellular carcinoma ( HCC ) and their correlation with tumor markers .Methods Th22 cells in blood samples from 45 patients with HCC and 15 normal controls were detected by flow cytometry ( FCM) .IL-22 levels were detected by an enzyme-linked immunosorbent assay .Statistical analysis of the relevance of Th 22 cells, IL-22 levels and tumor markers including AFP, CA125, CA193, CA199, CEA was performed.Results The level of Th22 cells and IL-22 in peripheral blood of patients with primary hepatic carcinoma were significantly higher than that of normal controls ( P<0.05), but no significant between male and female group .The percentage of Th22 cells in HCC patients had positive correlation with the level of IL-22.Th22 cells, IL-22 levels had no correlation with tumor markers including AFP , CA125, CA193, CA199, CEA(P>0.05).Conclusion The levels of Th22 cells and IL-22 expression in peripheral blood of HCC patients are high but have no correlation with tumor markers and their clinical significance in HCC still need large clinical sample analysis .