SELDI-TOF-MS 在筛选肝癌高发家系与无癌家系血清蛋白差异中的应用

摘要

Objective To compare serum protein pattern of patients with liver cancer from high-risk families for liver cancer with that of patients with liver cancer from no-risk familial pedigree for liver cancer and detect specific biomarkers in serum between two groups .Methods SELDI-TOF-MS Protein Chip was used to detect serum protein patterns of 25 patients with liver cancer from high-risk familial pedigree for liver cancer and 25 patients with liver cancer from no-risk families for liver cancer .Diagnostic model was developed and validated by using BPS .Results At the molecular weight range from 2 000 to 50 000 ,15 protein peaks were obviously different between two groups .Ana-lyzed by BPS, the diagnostic model′s accuracy was 92.0%(46/50),sensitivity was 96.0%(24/25) and specificity was 88.0%(22/25).Conclusion There are amount of the special serum differential proteins between two groups ,SELDI-TOF-MS Protein Chip array technology has better clinical value in detecting the special serum protein biomarkers .%目的：比较肝癌高发家系患者和无癌家系患者血清蛋白质谱图，探寻两者间的血清蛋白差异。方法用表面增强激光解吸电离-飞行时间质谱技术（ SELDI-TOF-MS）及CM10蛋白芯片对25例肝癌高发家系患者和25例无癌家系患者血清样本进行分析，将获得的血清蛋白质指纹图谱，用计算机软件进行比较分析，建立高发家系患者和无癌家系患者的比较筛选模型。结果肝癌高发家系组与无癌家系组共有15个蛋白质差异有统计学意义。筛选出4个蛋白峰建立诊断模型的灵敏度为96.0％（24/25），特异度为88.0％（22/25），准确率为92.0％（46/50）。结论肝癌高发家系患者组和无癌家系患者组的血清存在一定的差异蛋白， SELDI技术在特异性蛋白生物标志分子的筛选等方面具有较好的临床应用前景。