大鼠骨髓基质干细胞治疗C6胶质瘤实验研究

摘要

目的 观察骨髓基质干细胞(BMSCs)在体内环境下对C6胶质瘤的治疗效果.方法 流式细胞术检测培养的BMSCs表面标记;SD大鼠生存周期绘成Kaplan-Meier图,行Log-rank Test,HE 染色和BrdU免疫组化染色观察BMSCs对胶质瘤的趋向性和治疗情况.结果 流式实验显示所培养细胞为BMSCs;HE染色结果示呈瘤率为100％,实验组较对照组生存时间明显延长;BrdU免疫组化染色示BMSCs存在于肿瘤组织及其周边.结论 侧脑室移植BMSCs后,BMSCs可向肿瘤组织迁移,并可改善荷瘤大鼠的生存质量,明显延长其晚期生存周期.%Objective To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) for the treatment of C6 glioma in rats. Methods Flow cytometry was used to detect the surface markers of BMSCs. The survival time of SD rats was performed and Kaplan-Meier plot was generated, and then Log-rank Test was performed. HE staining and immnohistochemical staining of BrdU were used to investigate the treatment of BMSCs and their tropism to giloma. Results The results of flow cytometry showed that cultured cells were all BMSCs. The success ratio of tumor models was 100% detected by HE staining. The survival time in experiment group was significantly longer than that in control group. The immnohistochemical staining of BrdU showed BMSCs in and around the tumor tissues. Conclusion After transplantation of BMSCs in the lateral cerebral ventricles, BMSCs can migrate to tumor tissues. BMSCs can improve the life quality and prolong the survival time of glioma-bearing rats.