人脑胶质瘤中垂体瘤转化基因的表达及其意义

摘要

Objective To explore the expression of PTTG in human glioma cell of the patients with neurogliocytoma and to see whether the PTTG's expression was associated with the differentiation and pathologic grading. It was expected that this study could reveal some essential qualities of the glioma and provide potential application for judging the malignant grades and biological treatment. Method The expression of PTTG and PTTG mRNA was detected in 65 cases of human glioma by hemi-quantitative reverse transcription polymerase chain reaction(RT-PCR) and Western-blot analysis respectively. Results (1)In human glioma samples, the positive rate of PTTG mRNA and PTTG expression was 100%. (2)The gray degree ratios of PTTG mRNA belt to β-actin mRNA belt varied accordingly to different pathologic grades. There was significant difference between grade Ⅰ and grade Ⅲ or gradeⅣ(P<0.01). The same significance was also found between grade Ⅱ and grade Ⅲ or grade Ⅳ(P<0.01). The expressions of PTTG mRNA in grade Ⅲ or grade Ⅳ were significantly more than those of grade Ⅰ or grade Ⅱ. There was significant difference in the Reinhoit Zahl of PTTG belt to β-actin belt among groups (all P<0.01). (3)Analyses of correlation: The positive correlation existed between the expressions of PTTG or PTTG mRNA and the pathologic grading of human glioma(P<0.001). Conclusions (1) Detecting PTTG in human glioma can be used in diagnosing, judging the malignancy degree and the prognosis. (2) Detecting PTrG mRNA as molecular pathology in human glioma would retrieve the limitations of traditional tissue pathology. It will be an important cell marker that would act as more substantial role in future study.%目的 探讨垂体瘤转化基因(PTTG)在胶质瘤的诊断及恶性程度和预后判断中的临床意义.方法 采用半定量逆转录聚合酶链反应法(RT-PCR)和蛋白免疫印迹法(Western-blot)对65例胶质瘤患者进行PTTG mRNA和PTTG蛋白检测.结果 (1)65例胶质瘤组织中PTTG蛋白和PTTG mRNA表达全部呈阳性.(2)计算分组的标本PTTG mRNA和与β-actin mRNA灰度比值:Ⅰ级和Ⅲ、Ⅳ级之间差别有统计学意义(P<0.01),Ⅲ、Ⅳ级之间PTTG mRNA表达均高于Ⅰ级;Ⅱ级和Ⅲ、Ⅳ级之间差别有统计学意义(P<0.01),Ⅲ、Ⅳ级之间PTTG mRNA表达均高于Ⅱ级.计算分组的标本PTTG蛋白和与β-actin吸光度比值:四组之间任意两两比较,差别均有统计学意义(P<0.01).(3)相关性分析:PTTG蛋白和PTTG mRNA表达水平与胶质瘤病理分级均呈正相关性(P<0.001).结论 (1)PTFG的mRNA和蛋白的表达与胶质瘤病理分级呈正相关.检测FTTG的mRNA和(或)蛋白的表达,可以作为胶质瘤诊断、判断预后的一个可靠指标.(2)检测PTTG的mRNA的表达,从分子病理方面将可以弥补传统病理的局限性,在脑肿瘤的诊断、治疗和预后评估中将发挥重大的作用.