一种大脑皮层神经元特异性标记物的研究

摘要

Objective To study the characteristics of a new neuron-specific marker in the cerebral cortex and its application method.Methods According to immunofluorescence principles,the neurons in the cerebral cortex cultured in vitro were stained by fluorescein isothiocyanate (FITC)-labeled Tet1; and FITC-Tet1 with NSE or GFAP was employed to determine whether the neurons can be marked and whether they can be distinguished from astrocytes; the conditions of application of FITC-Tet1 were quantified.The HEK and C6 cell lines were performed the above experiments as controls.Results The final concentration was the key factor,which indicated that when the FITC-Tet1 was about 30 μg/mL,the neuron marking ability was the best,and the astrocytes,HEK and C6 were not stained.Neither high (100μg/mL) or low (10μg/mL) levels of FITC-Tet1 were suitableforstainingneurons.Conclusion Tet1 can specifically combine with the neuron in the cerebral cortex,which may make it to be a new staining method or a targeting molecule to neurons in the central nervous system.%目的 探讨一种新型大脑皮层神经元特异性结合物的特性及应用条件. 方法 将接有荧光素FITC的肽段Tet1通过免疫荧光技术对体外培养的大鼠大脑皮层神经元进行染色,免疫双标法检测FITC-Tet1特异性标记神经元能力,并对其应用条件进行量化.同时以HEK及C6细胞做为对照. 结果 FITC-Tet1达到一定浓度(30 μg/mL)时能特异性显示大脑皮层神经元,其几乎不与胶质细胞、HEK及C6细胞结合.过高(100 μg/mL)或过低(10 μg/mL)浓度均不利于神经元标记. 结论 Tet1能特异性结合大脑皮层神经元,该肽段可能成为一种新型的中枢神经元标记或靶向手段.