目的 监测模拟480 m氦氧饱和潜水实验舱密闭环境微生物污染水平,为合理有效评估舱室环境中微生物对作业人员健康的危害程度,制定科学的干预措施提供依据.方法 用自然沉降法监测环境微生物浓度,用选择性培养基筛查污染指示菌生长特点,用VITKE2微生物自动鉴定系统检测微生物种类,以国家普通室内微牛物污染评价标准评价舱内微生物污染程度.通过生长曲线和运动能力测定环境对优势致病菌铜绿假单胞菌毒力相关生物学特性的影响.结果 实验过程中饱和舱微生物监测浓度超国家评价标准3.9 ～8.4倍,微生物种类以人源性条件致病菌为主,优势菌铜绿假单胞菌生长和运动能力明显增强.结论 模拟480 m氦氧饱和潜水实验舱微生物污染程度较重,氦氧饱和环境对铜绿假单胞菌致病力有明显诱导作用,致病微生物是该环境中潜在的健康损害因素.%Objective To monitor and evaluate the airborne microbial pollution in the confined environment of the 480 m saturation diving chamber and to provide evidences for a reliable evaluation of the health hazard caused by microbes in the chamber environment,as well as for the development of scientific intervention measures.Methods The level of microbes in the environment was measured with the natural sedimentation method,the growing features of indicator microbes were screened with selected medium,types of isolated strains were identified with the VITKE2 system,and airbome microbial contents in the chamber were evaluated with the General National Indoor Microbe Pollution Evaluation Standards.The effect of the environment on the related biological features of the virulence of Pseudomonas aeruginosa,the dominant pathogen in the chamber was monitored by means of the growing curve and motor ability.Results As measured in the experiment,the level of airborne microbes in the saturation diving chamber was 3.9 - 8.4 times higher than that of the national evaluation standards.Microbial types were dominantly human origin pathogens.The growth and motor ability of the prevailing Pseudomonas aeruginosa were enhanced,when compared to the control groups.Conclusions Microbial pollution in the 480 m saturation diving chamber was serious,heliox saturation could induce or enhance the virulence of Pseudomonas aeruginosa,and pathogenic microbes were a potential health hazard to divers in the hyperbaric environment.
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