Objective To explore the isolation, culture and drug sensitivety test of Stephanoascus ciferrii, so as to provide the correct basis for clinical diagnosis and treatment.Methods Two strains of Stephanoascus ciferrii from one patient with chronic suppurative otitis media and another patient with secondary biliary tract infection after liver transplantation were isolated and cultured by conventional methods.The colony characteristics and morphology of the fungi were observed.Vitek 2Compact automatic microbiological analysis system was used for biochemical identification, and molecular identification was carried out in combination with ITS region sequencing.ATB FUNGUS 3was used for drug sensitivity test.Results The isolates could grow on the common culture medium of the blood plate, CHROMagar and sabourauad plate.The colony was not easy to be scraped from the agar.With the extension of culture time, the colony color gradually became blue, the colony began to be rough and the center was obviously hollow.The spores and mycelium were observed under microscopy.The fungal spores were ovate and arranged along the mycelium.The isolates were identified by Vitek 2Compact as the Stephanoascus ciferrii, and finally being comfirmed by molecular biological methods (similar to 100％).The results showed that both strains were sensitive to 5-fluorocytosine, amphotericin B, fluconazole, itraconazole and voriconazole.Conclusion Stephanoascus ciferrii is a rare opportunistic pathogenic fungus.Clinical laboratory traditional culture method combined with new molecular biology techniques, might quickly provide the accurate basis for clinical medication.%目的 了解西弗射盾子囊霉的分离培养鉴定和药敏试验, 为临床诊疗提供正确的用药依据.方法 采用常规方法分离培养来自于慢性化脓性中耳炎患者中耳分泌物与肝移植术后继发胆道感染患者胆汁标本的2例西弗射盾子囊霉, 观察真菌的菌落特征和镜下形态;采用Vitek 2Compact全自动微生物分析系统进行生化鉴定, 并结合ITS区测序进行分子鉴定;采用ATB FUNGUS 3进行药敏试验.结果 分离菌在哥伦比亚血琼脂平板、科玛嘉显色平板和沙保弱斜面培养基上均能生长, 呈镶嵌平板生长, 不易刮起;随着培养时间的延长, 菌落颜色逐渐变蓝, 菌落开始粗糙, 中心明显凹陷;镜下特征为孢子和菌丝可见, 真菌孢子为卵圆形、可沿菌丝排列;经Vitek 2Compact鉴定为西弗射盾子囊霉, 并经分子生物学方法确认 (相似度100％);药敏结果显示, 2例菌株均对5-氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑和伏立康唑敏感.结论 西弗射盾子囊霉是一种少见的机会致病性真菌, 临床实验室应将传统培养法与分子生物学技术相结合, 准确快速的为临床用药提供依据.
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