目的 分析自慢性化脓性中耳炎患者中耳分泌物分离的西弗射盾子囊霉的实验室检查特征,为少见真菌的诊治提供依据.方法 纳入西弗射盾子囊霉相关慢性化脓性中耳炎患者12例.培养其中耳分泌物,观察真菌的菌落特征和镜下特征,采用Vitek 2 Compact全自动微生物分析系统进行菌种鉴定.结果 西弗射盾子囊霉在血平板、巧克力平板和沙氏平板等临床常用培养基上均可生长,且在28℃和35℃均生长较好.培养1周后菌落呈奶油色、镶嵌生长,质地硬,周边呈花边样.该菌在科玛嘉念珠菌显色培养基上为中央蓝色、边缘白色的凸起小菌落.镜下特征为孢子和菌丝同时可见,小培养观察时可见菌丝有分隔,真菌孢子呈芽生型、形态卵圆、部分可沿菌丝排列.西弗射盾子囊霉可同化利用许多碳水化合物和常见氨基酸.用Vitek 2 Compact全自动微生物分析系统可鉴定该菌.结论 西弗射盾子囊霉是一种非常少见的机会致病性真菌,临床实验室应重视中耳分泌物中此真菌的培养鉴定.%Objective To study the laboratory features of Stephanoascus ciferrii isolated from middle ear excretion of the patients with chronic suppurative otitis media for providing the basis of diagnosis for the rare fungus infection.Methods The study was performed for 12 patients who were suffered from chronic suppurative otitis media associated with Stephanoascus ciferrii.The secretions of middle ear were cultured and the macroscopic features of colony and cells of the fungi were observed.The species of the fungi was identified by Vitek 2 Compact system.Results Stephanoascus ciferrii may grow on common media such as blood plate,chocolate plate and sabourauad plate,at 28 to 35 ℃.The form of the colonies displayed cream-colored,rough,hard and wrinkled quality after 1 week incubation.On CHROMagar medium the colonies looked like small mushroom with blue center and white edge.Under microscopy,abundant branched septate mycelium with small ramified chains of oval blastoconidia and the conidia were arranged along the side of hyphae.The fungi strain may assimilate lots of carbohydrates and common amino acid.Vitek 2 Compact automated system should be able to identify Stephanoascus ciferrii.Conclusion Stephanoascus ciferrii is a rare opportunistic pathogenic fungus.In clinical laboratories special attentions should be paid to culture and identification of the fungus from middle ear excretion.
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