小鼠烟曲霉角膜炎的实验研究

摘要

Objectives To compare the antifungal activity of itraconazole against Aspergillus fumigatus with fluconazole in vitro and to evaluate the therapeutic efficacy of itraconazole against Aspergillus fumigatus keratitis in mice. Methods Mice model of Aspergillus fumigatus keratitis was set up by intrastromal injection and confirmed by microscopy and fungal culture. Minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of itraconazole and fluconazole to Aspergillus fumigatus were determined by agar dilution method. Mice with Aspergillus fumigatus keratitis were treated by itraconazole. Clinical assessment, inflammatory evaluation and colony-forming unit (CFU) counting were recorded by the end of the treatment. Results The MICs and MFCs of itraconzole and fluconazole to Aspergillus fumigatus were 6.25 μg/mL and 12.5 )μg/mL, 500 μg/mL and 1 000 μg/mL, respectively. Compared with control group, mice treated by itraconazole showed significant improvement in clinical assessment, inflammatory evaluation and CFU counting ( P ＜ 0. 05). Conclusions Itraconzole is superior to fluconazole in the treatment of Aspergillus fumigatus keratitis in vitro .%目的 比较伊曲康唑和氟康唑对烟曲霉的体外抗菌活性,观察伊曲康唑对小鼠烟曲霉角膜炎的治疗作用.方法 通过角膜基质注射法建立烟曲霉角膜炎小鼠模型.造模后观察角膜病变,取角膜病变处分泌物做真菌镜检、真菌培养以证实造模成功.用药基法检测伊曲康唑和氟康唑对烟曲霉的最低抑菌浓度( MIC)和最低杀菌浓度(MFC).对烟曲霉角膜炎小鼠给予伊曲康唑治疗,治疗结束行临床评分、炎性评分、菌落形成单位测定以评价疗效.结果 伊曲康唑对烟曲霉的MIC和MFC分别为6.25 μg/mL、12.5 μg/mL;氟康唑对烟曲霉的MIC和MFC分别为500 μg/mL、1 000 μg/mL.伊曲康唑治疗组临床评分、炎性评分和测定的菌落数较对照组均明显减少(P＜0.05).结论 伊曲康唑对烟曲霉的体外抗菌活性优于氟康唑,并且对烟曲霉性角膜炎有明显疗效.