Objective To investigate the in vitro acvtivity of posaconazole against 43 clinical isolates of zygomycetous fungi. Methods Forty-three clinical strains were re-identified by ITS region sequencing. The minimal inhibitory concentration of posaconazole against 43 clinical isolates of zygomycetous fungi was assayed by E-test. Results Forty-three strains included Rhizopus microsporus (12 isolates) , Mucor irregularis (12 isolates) , R. oryzae (10 isolates) , Lichtheimia ramosa (3 isolates) , L. ornata (1 isolate) , Syncephalastrum racemosum (1 isolate) , Rhizomucor pusillus (1 isolate) , M. circinelloides (1 isolate) , M. indicus (1 i-solate) anAM. hiemalis ( 1 isolate). The MIC ranges of posconazole against Mucor spp. , Rhizopus spp. and Lichtheimia spp. in E-test were 0. 19-> 32 μg/mL, 0. 19-3 μg/mL and 0. 002-0. 38 μg/mL, respectively. The MIC values of posaconazole against Syncephalastrum racemosum and Rhizomucor pusillus were 1 μg/mL and 0. 19 μg/mL, respectively. Conclusion Most isolates of zygomycetous fungi were susceptible to posaconazole. The MIC values of Mucor spp. were relatively variable.%目的 研究白沙康唑对临床来源的接合菌体外抗菌活性.方法 对临床来源的43株接合菌采用ITS区序列分析进行准确鉴定,采用E-test纸片法研究泊沙康唑对43株菌的体外药物敏感性,观察其MIC值.结果 经ITS序列分析鉴定,43株临床来源接合菌中最为常见的是小孢根霉和不规则毛霉(各12株),其次为米根霉(10株),ramosa(3株).其他菌种分别为ornata、总状共头霉、微小根毛霉、卷曲毛霉、印度毛霉和冻土毛霉各1株.E-test纸片法测得泊沙康唑对毛霉属、根霉属和Lichtheimia spp.的MIC值范围分别为0.19～＞32 μg/mL、0.19～3 μg/mL、0.002～0.38 μg/mL,对总状共头霉和微小根毛霉的MIC值分别为1μg/mL和0.19 μg/mL.结论 泊沙康唑对大部分接合菌有效,不同种属MIC值范围不同,毛霉属真菌的MIC值差异较大.
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