1株致体股癣的颗粒型红色毛癣菌变种的形态学观察与基因鉴定

摘要

目的：探讨1株致体股癣的颗粒型红色毛癣菌变种的形态学特点及核糖体基因序列变化。方法1例体股癣患者皮损中分离出1株红色毛癣菌，观察形态学特点，并用PCR测定核糖体转录间区（ ITS）、D1/D2区、非转录区基因序列（ NTS区）内串联重复亚单位（ TRS）。结果该菌株可产生鹿角形菌丝，大分生孢子有中空现象及单侧出芽产孢特征，可在42℃生长；ITS和D1/D2区基因测序鉴定为红色毛癣菌，但TRS-1和TRS-2基因序列分别有2个、1个碱基差异。结论该菌株可能是颗粒型红色毛癣菌的1个变种。%Objective To investigate the morphological features and sequences of ribosomal genes in a variant of granular type Trichophyton rubrum isolated from tinea corporis and cruris Methods The morphological features of one T. rubrum strain recovered from a patient with tinea corporis and cruris were observed The ribosomal internal transcribed spacer( ITS),D1/D2 and non-tran-scribed region were determined by PCR Results The phenotype of the isolate produced antlers mycelium,macroconidium had hol-low phenomenon,unilateral budding and it grew at 42℃ The sequences of ITS and D1/D2 were identical to T. rubrum in the Gen-Bank ,but sequences of TRS-1 and TRS-2 in the non-transcribed region were different from the reference strains by 2 and 1 base,re-spectively Conclusion The strain might be a variant of granular type T. rubrum.