通脉刺五加胶囊对急性心肌缺血大鼠血流动力学和心电图的影响

摘要

目的：探讨急性心肌缺血对大鼠血流动力学和心电图J点位移的影响，以及通脉刺五加胶囊的干预作用。方法将30只筛选后的大鼠，按照体质量区域化随机分为3组（每组10只），分别为假手术组、模型组和给药组，给药组大鼠每日灌胃给予刺五加胶囊的混悬液3.5ml/kg，假手术组、模型组大鼠每日灌胃相同体积的生理盐水，连续给药14d。结扎大鼠左冠状动脉的前降支，复制大鼠急性心肌缺血的模型，将压力换能器导管自右侧的颈总动脉插入左心室，记录冠状动脉结扎后15min大鼠左心室压力的变化情况。同时，运用多道生理记录仪对大鼠的心电图（ECG）进行监测，分别记录大鼠冠状动脉结扎后0.5，1，2，4和6 h ECG中J点的位移（⊿J），来探讨通脉刺五加胶囊对急性心肌缺血的缓解作用。结果实验结果表明，与假手术组相比，模型组大鼠左心室舒张末期压（LVEDP）显著升高（P＜0.05），而左心室收缩峰压（LVSP）和压力最大变化率（±dp/dtmax）则显著降低（P＜0.05），且冠状动脉结扎后0.5，1，2，4和6 h ECG中⊿J明显升高（P＜0.05）。与模型组相比，给药组大鼠LVEDP明显下降（P＜0.05）， LVSP和±dp/dtmax明显升高（P＜0.05），且除0.5和6h两个时间点外（P＞0.05），其余各时间点ECG的⊿J均显著下降（P＜0.05）。结论大鼠左冠状动脉前降支结扎后，心肌收缩−舒张功能明显减弱，ECG中J点位移明显，通脉刺五加胶囊能明显缓解急性心肌缺血对大鼠心电图造成的影响。%ObjectiveTo determine the effect of acute myocardial ischemia on hemodynamic status and electrocardiogram (ECG) J-point displacement (⊿J) in rats and the intervention effect of Tongmai Ciwujia Capsule [a capsule mainly containing stem-leaf total flavonoid of Ciwujia, (Acanthopanax senticosus)] in the process.Methods Thirty rats with normal ECG were randomly divided into 3 groups according to the range of body mass, ie, sham operation group, model group and intervention group. The rats of the intervention group were orally administrated with Ciwujia suspension of 3.5ml/kg every day while the rats in the other 2 groups were given equal volume of normal saline for 14 consecutive days. Left anterior descending coronary artery (LAD) was ligated to establish the model of acute myocardial ischemia. The catheter of pressure transducer was put into the left ventricle to detect the changes of pressure in 15min after ligation. ECG was also monitored and⊿J was recorded in 0.5, 1, 2, 4 and 6h after ligation to evaluate the intervention of Ciwujia Capsule.Results Compared with sham operation group, left ventricular end-diastolic pressure (LVEDP) and ECG⊿J at 0.5, 1, 2, 4 and 6h after ligation were significantly increased (P<0.05), while left ventricular systolic peak pressure (LVSP) and maximum rate of pressure change (±dp/dtmax) were decreased markedly (P<0.05) in model group. Compared with model group, Ciwujia intervention resulted in significantly decreased LVEDP and⊿J at the above time points except in 0.5 and 6 h after ligation (P<0.05), and markedly increased LVSP and±dp/dtmax (P<0.05).Conclusion Ligation of LAD results in obviously impaired myocardial diastolic and systolic function and obvious J-point displacement ECG. Tongmai Ciwujia Capsule could effectively alleviate the impact of acute myocardial ischemia on ECG of rats.