视网膜母细胞瘤和正常视网膜组织中VEGF-C表达和淋巴管计数的研究

摘要

目的 研究视网膜母细胞瘤(retinoblastoma,RB)和正常视网膜组织中血管内皮生长因子-C(vascular endothelial growth factor-C,VEGF-C)的表达和淋巴管计数,探讨其相互关系及意义. 方法 收集30例RB眼球摘除手术标本和15例正常视网膜组织标本,采用Envision免疫组织化学法行VEGF-C和淋巴管染色. 结果 RB组织中VEGF-C表达阳性率及淋巴管计数明显高于正常视网膜组织(P＜0.01)；临床分期Ⅰ期、分化型、未侵犯视神经及生存期≥2年RB病例VEGF-C表达阳性率及淋巴管计数明显低于临床分期Ⅲ期、未分化型、侵犯视神经和生存＜2年病例(P ＜0.05或P＜0.01).VEGF-C阳性表达RB中淋巴管计数明显高于阴性病例(P＜0.01). 结论 VEGF-C表达水平和淋巴管计数可能是反映RB进展、生物学行为和预后的标记物,VEGF-C可能具有促进RB组织中淋巴管生成作用.%Objective To investigate the VEGF-C expression and lymph vessel counts in retinoblastoma (RB) and normal retina tissue, to study their interrelationship and clinicopathological significance. Method Envision immunohistochemistry stainings of VEGF-C and D2-40 were performed in 30 retinoblastoma and 15 normal retina tissues, which was routinely paraffin imbedded. Results The positive rate of VEGF-C expression and lymph vessel counts were higher in RB tissue than normal control (P<0.01) ; Samples from the clinical stage Ⅰ, differentiated type, uninvolved optic nerve and life span ≥2 year cases had higher expression of VEGF-C and higher lymph vessel counts than those from clinical stage Ⅲ , undifferentiated type, involved optic nerve and life span < 2 year cases( P <0.05 or P <0.01). Lymph vessel counts in VEGF-C positive samples were much higher than negative ones. Conclusion VEGF-C expression and lymph vessel counts maybe useful markers of RB progression , biological behavior and prognosis; VEGF-C might have promotive effect on lymphangiogenesis in RB tissue.