MVD在子宫内膜癌变过程中的表达及意义

摘要

目的：研究微血管密度(MVD)在子宫内膜癌变过程中的表达及意义,探讨血管生成对子宫内膜癌发生、发展的影响。方法应用CD105单克隆抗体,通过免疫组织化学SP法标记正常增生期子宫内膜组(20例)、子宫内膜不典型增生组(20例)及子宫内膜腺癌组(26例)中的血管内皮细胞,再结合图像分析技术进行MVD计数。结果 CD105-MVD值在正常增生期子宫内膜组(11.35±2.37)、子宫内膜不典型增生组(22.57±4.92)及子宫内膜腺癌组(27.66±2.31)中逐渐递增,三组比较差异具有统计学意义(F=137.969, P<0.01)；组间两两比较差异均具有统计学意义(P<0.01)。结论肿瘤血管形成参与了子宫内膜癌发生、发展、浸润和转移的过程。%ObjectiveTo research expression and significance by microvessel density (MVD) in endometrial cancerization, and to investigate influence by angiogenesis on occurrence and development of endometrial carcinoma.MethodsVascular endothelial cells in endometrium of normal proliferative stage group (20 cases), atypical hyperplasia of endometrium group (20 cases), and endometrial adenocarcinoma group (26 cases) were marked by CD105 monoclonal antibody through immunohistochemical SP method. Further MVD count was made in combination with image analysis technique.ResultsCD105-MVD value was gradually decreased from (11.35±2.37) in endometrium of normal proliferative stage group, to (22.57±4.92) in atypical hyperplasia of endometrium group and (27.66±2.31) in endometrial adenocarcinoma group. Their difference had statistical significance across the three gorups (F=137.969,P<0.01), and the differences between each two groups all had statistical significance (P<0.01).ConclusionTumor angiogenesis is involved in occurrence, development, infiltration and metastasis of endometrial carcinoma.