反相高效液相色谱法测定盐酸乙哌立松片剂的含量

摘要

目的：建立高效液相色谱法测定盐酸乙哌立松片含量的方法。方法：盐酸乙哌立松片加甲醇、超声振荡、过滤后以ODS-C18为分析柱，以甲醇-水-三乙胺(80∶19.5∶0.5，V/V)为流动相，托哌酮为内标，于254nm波长检测。结果：盐酸乙哌立松在0.4～1.6mg/ml浓度范围内线性关系良好(r=0.9999)，平均回收率98.4%，RSD=1.2%。三批盐酸乙哌立松片含量测定结果分别为标示量的97.8%，98.3%和98.9%。结论：方法快速、准确、专一性强，适用于盐酸乙哌立松片的含量测定。%OBJECTIVE：To develop a method for the determination of eperisone hydrochloride by high performance liquid chromatography(HPLC).METHOD:C18 column as the stationary phase and methanol-water-triethylamine(80∶19.5∶0.5,V/V,adjusted to pH 7.5 with glacial acetic acid) as the mobile phase were adopted.The detection was performed at 254nm.Eperisone hydrochloride tablets were disssolved in methanol,supersonic-vibrated and filtered.Tolperisone was used as internal standard.RESULTS:The standard curve was linear in the range of 0.4～1.6mg/ml with the excellent correlation coefficient of 0.9999.The average recovery was 98.4%(RSD=1.2%,n=15).Three batches of eperison hydrochloride tablets were analyzed and the labelled contents were 97.8%,98.3% and 98.9% respectively.CONCLUSION:The method was simple,sensitive,rapid and suitable for the quantitative anylysis of the preparation.