浆液性和黏液性卵巢囊腺癌患者线粒体结构和紧密连接蛋白表达的差异比较∗

摘要

目的：：探讨浆液性卵巢囊腺癌(SC)与黏液性卵巢囊腺癌(MC)细胞线粒体结构及紧密连接(TJ)蛋白occludin/ZO-1表达异常的差异及可能机制。方法：临床确诊30例卵巢囊腺癌患者中15例为 SC(SC 组),15例为MC(MC 组),另选3例子宫肌瘤患者进行对照(对照组)。采用电镜观察各组线粒体超微结构；采用体视学方法分析线粒体面数密度(Nm)、体密度(Vvm)和外膜面密度(Sv)；采用免疫组化方法检测各组卵巢上皮细胞 occludin/ZO-1蛋白的阳性单位(PU)及其强弱程度(用积分光密度值 IOD 表示)。结果：电镜下,对照组线粒体及内质网均未见异常；MC 组线粒体体积小、数量较多,线粒体 Nm、Vvm 和 Sv 较对照组明显降低(P <0.05)；SC 组线粒体体积大,脊断裂显著,线粒体 Nm、Vvm 和 Sv 较 MC 组显著升高(P <0.01)。免疫组化分析显示 MC 组 occludin 和ZO-1 PU 明显高于对照组(24.23±4.74 Vs 21.07±5.51、37.07±3.58 Vs 20.46±4.89,P 均<0.05),IOD 明显低于对照组(72.39±9.86 Vs 92.38±7.17、73.51±6.86 Vs 80.34±7.04,P 均<0.05)；SC 组 occludin 和 ZO-1 PU明显低于对照组(11.50±4.15 Vs 21.07±5.51、16.24±3.61 Vs 20.46±4.89,P 均<0.05),IOD 明显高于对照组(101.38±6.16 Vs 92.38±7.1、92.78±3.07 Vs 80.34±7.04,P 均<0.01)。结论：SC 及 MC 两种卵巢囊腺癌患者线粒体结构和体视学特征明显不同,可能与 TJ 蛋白的 occludin/ZO-1表达异常有关。%Objective:To investigate the mechanisms and the abnormal differences of mitochondrial construc-tion and the tight junction (TJ)protein occludin/ZO-1 expression in serous ovarian cystadenocarcinoma (SC)and mucinous ovarian cystadenocarcinoma (MC).Method:30 patients with ovarian cystadenocarcinoma in 1 5 cases for SC group (SC group),1 5 cases of MC (MC),an alternate 3 cases of myoma of uterus controls (control group). The mitochondrial ultrastructure were observed by electron microscope.The number density of mitochondria (Nm), volume density (Vvm)and the outer membrane surface density (Sv)were detected by the stereology analytical method.The ovarian epithelial cell protein positive unit(PU)of occludin/ZO-1 and its power degree (expressed in in-tegral density,IOD)of each group were detected by immunohistochemical method.Results:Under electron micros-copy,there were no abnormal mitochondria and endoplasmic reticulum in the control group.Mitochondria of MC group was small in size,quantity was more,the mitochondria Nm,Vvm and Sv significantly lower than the control group (P < 0.05);SC group,mitochondrial volume,spinal fracture significantly,mitochondria Nm,Vvm and SvMC group significantly increased (P < 0.01 ).Immunohistochemical analysis showed that positive rate of occludin and ZO-1 in MC group was obviously higher than that of control group(24.23±4.74PU Vs 21.07±5.5 1PU,37.07 ±3.58PU Vs 20.46 ±4.89PU,P <0.05),IOD significantly lower than the control group (72.39±9.86 Vs 92.38 ±7.1 7,73.5 1±6.86 Vs 80.34±7.04,P <0.05).Occludin and ZO-1 in SC group were significantly lower than the control group(1 1.50±4.1 5PU Vs 21.07±5.5 1PU,1 6.24±3.61PU Vs 20.46 ±4.89PU,P <0.05),IOD was sig-nificantly higher than the control group(101.38±6.1 6 Vs 92.38±7.1,92.78 ±3.07 Vs 80.34±7.04,P <0.01). Conclusion:The mitochondria stereology characteristics of patients with SC and MC are obviously different,may be related to express abnormalities of occludin/ZO-1 with TJ proteins.