外源性IL-10对变应性鼻炎大鼠Th细胞和Th17细胞相关因子表达的影响

摘要

Objective To investigate the expression of IFN-γ, IL-5, IL-17 and TGF-βby Th cells and Th17 cells in rats with allergic rhinitis upon the intervention of IL-10.Methods SD rats were ran-domly divided into three groups including allergic rhinitis ( AR ) group, IL-10 treated group and control group (n=10).Rats in AR group and IL-10 treated group were sensitized by injection of ovalbumin (OVA) and aluminum hydroxide on the 1st, the 7th and the 14th days.The rats treated with equal volume of saline were set up as the control.The corresponding interventions ( OVA, OVA and IL-10, saline) were respec-tively given to rats in each group on the 21th day for 7 consecutive days.The clinical manifestations in rats were observed within 30 minutes after each administration.Serum samples were collected at 48 hours after the last challenge for the detection of IgE and OVA-sIgE.ELISA and Western blot assay were performed to detect IFN-γ, IL-5, IL-17 and TGF-βin nasal mucosa samples.Results Some characteristic symptoms of AR were observed in rats from AR group and IL-10 treated group.Compared with IL-10 treated rats, rats in AR group showed severe clinical symptoms such as constant rubbing and tearing of the eyes (P<0.05).The levels of IgE and OVA-sIgE in serum samples and the levels of IFN-γ, IL-5, IL-17 and TGF-βin nasal tis-sues were significantly increased in rats with RA (P<0.05), but were reduced with IL-10 intervention (P<0.05).Conclusion Exogenous IL-10 could be used to treat AR by reducing the expression of IFN-γ, IL-5, IL-17 and TGF-βin nasal tissues.%目的：探讨外源性白细胞介素-10（ IL-10）对变应性鼻炎大鼠（ AR） Th细胞和Th17细胞相关因子表达的影响。方法 SD大鼠30只，采用随机数字表法将其分为AR模型组、IL-10组和阴性对照组，每组10只。 AR组和IL-10组采用常规卵白蛋白（ OVA）联合氢氧化铝致敏法建立AR大鼠模型，阴性对照组采用生理盐水代替，IL-10组于激发21 d后，给予OVA的同时，腹腔注射40 pg／kg的IL-101 ml，连续7 d；各组于给药后30 min内观察各组大鼠临床表现，记录大鼠的行为学得分，并于最后一次激发后48 h，取血清检测IgE和OVA-sIgE含量，同时取大鼠双侧鼻腔呼吸区黏膜，用ELISA试剂盒检测IFN-γ、IL-5、IL-17和TGF-β含量变化，同时用Western blot法检测各指标的表达水平。结果 AR组大鼠均表现出四处摩擦、涕流满面等重度AR的临床表现，与其相比，IL-10组表现明显减轻（P＜0．05）；各组血清中IgE和OVA-sIgE含量及鼻黏膜中IFN-γ、IL-5、IL-17和TGF-β含量均明显升高（P＜0．05），经IL-10干预后，其含量明显降低，且与AR组相比，差异有统计学意义（P＜0．05）；Western blot结果显示，与对照组相比，AR组和IL-10组大鼠鼻黏膜中IFN-γ、IL-5、IL-17和TGF-β表达水平明显升高，IL-10组大鼠鼻黏膜中IFN-γ、IL-5、IL-17和TGF-β表达水平低于AR组（ P＜0．05）。结论用外源性IL-10的方法治疗AR，并降低鼻黏膜中IFN-γ、IL-5、IL-17和TGF-β的表达，可在一定程度上达到治疗AR的目的。