RBP4诱导HUVEC炎症反应作用机制的研究

摘要

目的：探讨血清视黄醇结合蛋白4(RBP4)诱导人脐静脉内皮细胞(HUVEC)炎症的作用机制。方法：采用MTT还原法检测细胞活性,通过DCFH-DA检测活性氧的生成,Western blotting分析相关蛋白的表达,凝胶滞留法(EMSA)分析NF-kB的DNA结合活性。结果：RBP4处理后,MTT检测发现人脐静脉内皮细胞存活率显著低于对照组,流式细胞仪的结果表明活性氧的水平也显著升高。结论：RBP4可诱导人脐静脉内皮细胞(HUVEC)毒性及炎症反应。%Objective:To investigate the mechanism of action of serum retinol binding protein 4 (RBP4) in human umbilical vein endothelial cells (HUVEC) induced inflammation. Methods:MTT reduction assay cell activity by DCFH-DA to detect reactive oxygen species generation, Western blotting analysis of related proteins, gel retardation (EMSA) analysis of NF-kB DNA binding activity. Results:RBP4 in treatment, MTT assay found that human umbilical vein endothelial cells was significantly lower than the control group, the flow cytometry results show that reactive oxygen species levels were also significantly elevated. Conclusion:RBP4 in human umbilical vein endothelial cells (HUVEC) could induce toxicity and inflammatory response.