大鼠清醒无麻醉状态下的侧脑室给药方法的研究

摘要

目的:探讨大鼠清醒无麻醉状态下的侧脑室给药方法.方法:选取雌性SD大鼠15只,使用自制脑室置管系统对大鼠行侧脑室置管,置管术后大鼠自由恢复1周,每日对大鼠进行驯养5～10 min.术后8～10d在清醒无麻醉状态下每日向侧脑室注射生理盐水10 μL.术后11d侧脑室注入10 μL美蓝后麻醉大鼠,全部开胸经升主动脉插管,行甲醛心脏灌注后处死大鼠,待甲醛固定灌注完毕后取脑组织.结果:2％的美蓝溶液注入后脑室的全部内壁均染色,苏木精-伊红染色法(HE染色法)染色后脑内未见明显的出血或炎症,2只大鼠给药前,置入侧脑室的不锈钢导管未见脑脊液溢出.结论:清醒无麻醉状态下的大鼠侧脑室给药方法简单可行,成功率高.%Objective:To investigate the method of intracerebroventricular administration of conscious rats without anesthesia.Methods:15 femaleSD rats were selected.The tube was placed in the lateral ventricles by the self-made ventricular catheterization system.After the catheterization,the rats recovered freely for 1 week,and the rats were tamed for 5 to 10 minutes every day.From 8 to 10 days after operation,10 μL physiological saline was injected into the lateral ventricle every day without anesthesia.On the 1 1 th day rats were anesthetized after 10 μL intracerebroventricular injection of methylene blue,open all chest and the rats were sacrificed by formaldehydeperfusion though the ascending aorta cannulation.After that the brain tissues were took out.Results:All inner wall of ventricle were stained after 2％ methylene blue was poured in.There was no obvious bleeding or inflammation after hematoxylin and eosin staining (HE staining) in the brain,There was no cerebrospinal fluid in stainless steel conduit placed in the lateral ventricle catheter of 2 rats before insertion.Conclusion:The method of intracerebroventricular administration of rats without anesthesia is simple and feasible,and the rate of success is high.