FePt/GO纳米复合材料在放化疗中的应用

摘要

Objective To explore the potential anticancer effect of FePt/graphene oxide (FePt/GO) nanocomposites in lung cancer.Methods FePt nanoparticles with particle size of about 3 nm were prepared by a chemical reduction method,and FePt/GO nanocomposites were facilely produced by assembling FePt nanoparticles to the surface of GO through sonicate.Transmission electron microscope,atom force microscope and X-ray photoelectron spectroscopy were utilized to characterize the surface morphology and chemical composition of FePt/GO nanocomposites.The obtained FePt/GO nanocomposites were cultured with non small cell lung cancer cells (H1975 cells) and Human embryonic lung fibroblasts (HELF cells),separately,and the cytotoxicity of FePt/GO nanocomposites was evaluated in H1975 cells and HELF cells by CCK-8 assay.The radiosensitization ability of FePt/GO nanocomposites was determined by colony formation test and CCK-8 assay.Results FePt/GO nanocomposites were successfully prepared in this study.Within a certain concentration range,FePt/GO nanocomposites showed significant toxicity to H1975 cells,while keeping no obvious toxicity to HELF cells.Furthermore,FePt/GO nanocomposites enhanced the suppression of X-ray on H1975 cells growth.Conclusion FePt/GO nanocomposite is a potential anticancer agent which could be used in clinical treatment.%目的:探索FePt/GO纳米复合材料的抗肺癌效果.方法:通过化学还原的方法制备出径粒约为3 nm的FePt纳米颗粒,并通过超声将FePt纳米颗粒装载于氧化石墨烯(GO)表面得到FePt/GO纳米复合材料,运用TEM、AFM、XPS等技术对其进行表征.将得到FePt/GO纳米复合材料分别与非小细胞肺癌细胞(H1975)和人胚肺成纤维细胞(HELF)共同培养,用CCK-8法对其进行毒性评估;通过单细胞克隆形成实验与CCK-8法评估FePt/GO复合纳米材料的放射增敏作用.结果:成功制备出FePt/GO纳米复合材料,在一定浓度范围内,FePt/GO对H1975细胞表现出很明显的毒性而对正常HELF细胞无明显毒性,并且可以有效地增强X射线对H1975细胞的杀伤作用.结论:FePt/GO纳米复合材料是一种有可能用于临床的抗癌药物.