胶原蛋白海绵浸提液对小鼠体外脾脏淋巴细胞转化作用的研究

摘要

Immunogenicity for medical devices of animal origin is the key and diffi cult point during immune safety evaluation for these devices. This paper firstly investigated the effect of colagen sponge of animal origin on mouse splenic lymphocyte transformation and proliferation, and then analyzed the infl uence factors on the MTT method and CFSE method. The results showed that colagen sponge extract cannot signifi cantly induce transformation and proliferation of mouse splenic lymphocyte in vitro.%动物源医疗器械的免疫原性是评价其免疫毒理学安全性的关键和难点。该文利用MTT法和CFSE法体外研究动物源性胶原蛋白海绵浸提液对小鼠脾脏淋巴细胞转化和增殖作用，并对两种方法的影响因素进行了分析。研究表明，胶原蛋白海绵浸提液未引起小鼠体外脾脏淋巴细胞发生明显转化和增殖。