STK39基因多态性与汉族原发性高血压患者氢氯噻嗪疗效的相关性研究

摘要

Objective To assess the association of single nucleotide polymorphisms (SNPs) of STK39 gene with response to hydrochlorothiazide among ethnic Han Chinese patients with essential hypertension.Methods In total 118 patients with essential hypertension were recruited.All participants had received six weeks of treatment with hydrochlorothiazide 25 mg daily.Blood pressure (BP) and heart rate (HR) were measurement every 2 weeks.Genotypes of STK39 rs3754777 and rs6749447 were determined using a SNaPshot technique.Results A significant difference was found in ΔSBP between individuals with rs3754777 CC,CT and TT and those with rs3754777 CC and CT-TT (P＜0.05).A significant difference was also detected in ΔDBP between those with rs3754777 CC and CT-TT (P＜ 0.05).No significant difference was found in ΔSBP and ΔDBP between individuals with STK39 rs6749447 GG,GT and TT (all P ＞0.05).Relative risk analysis showed that STK39 rs3754777 was significantly associated with BP response to hydrochlorothiazide (OR =0.416,95％ CI =0.189-0.918,P＜ 0.05).Conclusion Polymorphisms of STK39 rs3754777 may be associated with BP response to hydrochlorothiazide among ethnic Han Chinese with essential hypertension.%目的 探讨STK39基因单核苷酸多态性(single nucleotide polymorphism,SNP)与汉族原发性高血压患者氢氯噻嗪疗效的相关性.方法 选择轻中度原发性高血压患者118例,每日1次给予25 mg氢氯噻嗪片,连续服用6周,每两周随访1次,监测患者的血压、心率.采用SNaPshot SNP分型技术对STK39 rs3754777和rs6749447两个位点进行基因分型.结果 STK39 rs3754777 CC、CT与TT 3组之间及CC与CT-TT两组间ΔSBP比较,差异均有统计学意义(P值均＜0.05).STK39 rs3754777 CC与CT-TT两组基因型间ΔDBP比较差异有统计学意义(P＜0.05).STK39 rs6749447 GG、GT与TT各组基因型间ΔSBP和ΔDBP的比较差异均无统计学意义(P＞0.05).相对风险度(OR)分析显示,STK39rs3754777与氢氯噻嗪的降压效应显著相关(OR=0.416,95％ CI=0.189～0.918,P＜0.05).结论 STK39 rs3754777基因多态性可能与汉族原发性高血压患者使用氢氯噻嗪的降压效应有一定的相关性,而STK39 rs6749447与氢氯噻嗪的降压效应之间无明显的相关性.