小细胞肺癌血清细胞因子的表达及其诊断价值

摘要

Background and objective It has been proven that serum cytokines could be changed in the early stage of patients with small cell lung cancer (SCLC). The current research investigates the different changes of cytockines and searches for potential biomarkers to improve the situation of the early diagnosis of SCLC. Methods The differential expression cytokines were detected using Reybiotech G6/G7 analysis of microarrays in the serum of 4 SCLC patients, 4 normal controls, and 4 phlegmonosis controls. The differential cytokines were confirmed by enzyme-linked immunosorbentassay (ELISA) in 197 SCLC patients, 180 normal controls, and 97 phlegmonosis controls. Results The levels of the 4 most valuable biomarkers in SCLC, including Leptin, MSP-o, uPAR, and MIP-ljJ, were significantly higher than that in the other groups with microaaray screening (P<0.05). The ELISA results showed that the uPAR are much higher in SCLC patients than that in the controls, in which the diagnostic sensitivity and specificity were 52.93% and 83.36%, respectively. The Leptin level was significantly higher in SCLC patients who do not have obvious body weight lost, whereas no difference were found in the SCLC who have obvious body weight lost compared with control groups. The diagnostic sensitivity and specificity of Leptin are 50.11% and 86.77%. The MSP-α and MIP-1β level have no significant difference among the three groups. Conclusion Ihe uPAR elevated level is significant in indicating SCLC diagnoses. The Leptin may be associated with SCLC in patients who do not have a change in weight.%背景与目的 已有的研究表明:小细胞肺癌(small cell lung cancer,SCLC)患者可出现血清细胞因子表达水平的变化,本研究通过检测SCLC患者血清中细胞因子的差异改变,探讨其在SCLC中的诊断价值.方法 首先使用Reybiotech G6/G7细胞因子芯片对4例SCLC患者、4例健康人和4例炎症患者血清进行细胞因子差异表达筛查,进一步应用酶联免疫吸附法(ELISA试剂盒)对197例SCLC患者、180例正常对照血清以及97例炎性病变患者血清进行验证.结果 芯片检测120种细胞因子,从有明显差异的细胞因子中选出最有研究价值的4种进行验证,包括尿激酶型纤溶酶原激活剂受体(urokinase plasminogen activator receptor,uPAR)、人瘦素(Leptin)、巨噬细胞刺激蛋白(macrophage stimulating protein α,MSP-α)和巨噬细胞炎症蛋白1β(macrophage inflammatory protein 1β,MIP-1β).采用ELISA法验证上述结果,SCLC患者血清uPAR较健康人群以及炎症患者增高(P＜0.05),诊断的敏感度为52.93％,特异度为83.36％.Leptin在无体重变化SCLC组较健康人群以及炎症患者增高,诊断的敏感度为50.11％,特异度86.77％；而Leptin在体重下降组较对照组无明显差异.此外,血清MSP-α、MIP-1β水平在三组之间比较无明显差异.结论 血清uPAR升高在SCLC中具有一定的诊断价值,而Leptin在无体重变化的SCLC中可能具有诊断意义.