Objective To investigate the relationship between hepatic pathology and the expression of IL-12 and IL-18 in patients infected with HBV. Methods Total of 175 patients infected with HBV in the Second Affiliated Hospital of Anhui Medical University were enrolled. The clinical data, liver inflammation grades and fibrosis stages were assessed. Immunohistochemical method was used to detect the expression of IL-12 and IL-18 in liver tissues. Spearman rank correlation was used to analyze the correlations between IL-12 and IL-18 expression and the above indicators. Results IL-12 and IL-18 were mainly expressed in the cytoplasm of liver cells. The expression of IL-12 and IL-18 in patients with chronic hepatitis B, liver cirrhosis after hepatitis B and hepatitis B related liver cancer had statistically significant difference (χ2 = 38.390, P < 0.001;χ2 = 21.051, P = 0.002). The expression of IL-12 and IL-18 in liver tissues of patients infected with HBV were positively correlated with the degree of inflammation and fibrosis (r = 0.179, 0.165, 0.241, 0.132; P = 0.032, 0.026, 0.042, 0.0195). Conclusion The expression of IL-12 and IL-18 may be related to the immune pathogenesis of HBV infection.%目的 探讨HBV感染者肝组织中IL-12和IL-18的表达与炎症及纤维化分级间的相关性.方法 收集于安徽医科大学第二附属医院住院的175例HBV感染者的肝脏病理组织以及临床资料,采用半定量SP免疫组织化学法检测肝组织中IL-12和IL-18的表达,并采用Spearman相关分析探讨其与肝脏病理间的相关性.结果 IL-12和IL-18在肝组织中的表达部位以细胞质为主,其在慢性乙型肝炎、乙型肝炎后肝硬化和乙型肝炎相关肝癌患者肝组织中的表达有显著差异(χ2=38.390,P<0.001;χ2=21.051,P=0.002),且与肝组织炎症及纤维化程度呈正相关(r值分别为0.179、0.165、0.241和0.132,P值分别为0.032、0.026、0.042和0.019).结论 IL-12和IL-18在HBV感染后的免疫发病机制中发挥重要作用,参与HBV感染的慢性化和疾病的转归.
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