探讨常规HPV检测采用RNA优势于DNA检测的临床意义

摘要

目的 探讨常规HPV检测采用RNA优势于DNA检测的临床意义.方法 选取湖南省肿瘤医院检验科于2016年1月-2016年12月收治的因宫颈疾病行宫颈活检的200例患者资料.根据病理活检结果将其分为4组, 测定各组患者宫颈口及宫颈管脱落的上皮细胞HPV E6/E7 mRNA与HPV DNA的含量并统计阳性例数, 探究其用于诊断宫颈癌的的价值及意义.结果与对照组相比, 低级别组, 高级别组, 浸润癌组HPV E6/E7 mRNA与HPV DNA的阳性例数与拷贝数明显升高, 差异具有统计学意义 (P＜0.05) .与低级别组相比, 高级别组与浸润癌组HPV E6/E7 mRNA阳性例数与拷贝数明显升高 (P＜0.05), 高级别组与浸润癌组HPV DNA阳性例数与拷贝数无明显差异 (P＞0.05) .结论 相比于HPV DNA检测, HPV E6/E7 mRNA在不同级别的宫颈上皮内瘤变患者检测中具有更好的敏感性, 提示HPV E6/E7 mRNA更适用于评估宫颈疾病恶化风险.%Objective To investigate the clinical significance of HPV DNA detecting and HPV E6/E7 mRNA detecting.Methods 200 patients with cervical diseases treated by cervical biopsy were selected in Hu Nan Tumor Hospital, from January 2016 to December 2016.They were divided into 4 groups according to pathological results, HPV DNA and HPV E6/E7 mRNA of epithelial cells of cervix in each group were detected.To investigate the value and significance of these methods in the diagnosis of cervical cancer.Results Compared with the control group, the positive number and copy number of HPV E6/E7 mRNA and HPV DNA in the low grade group, advanced group and invasive cancer group were increased significantly (P＜0.05).Compared with the low grade group, the positive cases and copy number of HPV E6/E7 mRNA were significantly higher in advanced group and invasive cancer group (P＜0.05), the positive cases and copy number of HPVDNA were no significant difference (P＞0.05).Conclusion Compared with HPV DNA detecting, HPV E6/E7 mRNA have better sensitivity in patients with the low-grade cervical intraepithelial neoplasia.It Suggests that HPV E6/E7 mRNA is more appropriate for assessing the risk of worsening cervical disease.