EGF/Gastrin联用对实验性1型糖尿病大鼠胰岛β细胞再生的影响

摘要

目的 观察EGF/Gastrin联用对实验性1型糖尿病大鼠胰岛β细胞再生的影响,为糖尿病的治疗开辟新途径.方法 选用Wister大鼠,一次性腹腔注射STZ(55 mg.kg-1)诱导1型糖尿病模型.实验分3组:正常对照组、糖尿病组及EGF/Gastrin组.EGF(1 μg.kg-1)/Gastrin(3 μg.kg-1)每日一次,连续14日.实验结束检测各组大鼠的空腹血糖、血清Insulin及C肽等生化指标;进行HE染色,观察胰岛形态学变化.结果 EGF/Gastrin组大鼠空腹血糖明显低于糖尿病组(P＜0.05);血清Insulin、C肽含量及胰岛β细胞数量均明显高于糖尿病组(P＜0.05).结论 EGF/Gastrin联用能够降低糖尿病大鼠的血糖,并使血清Insulin 和C肽含量增加,胰岛β细胞数量增多,功能得到改善.%Objective 　Purpose To observe the effects of combined use of EGF and Gastrin on the pancreatic β cell regeneration in type 1 diabetes experimental rats and find new methods to treat type diabetes .Methods 　STZ （55m g ·kg - 1 ）solution was intraperitoneal injected once on the Wister rats to creat the type 1 diabetes rats model .Rats were devided into 3 groups which were labeled Control Group ，DM Group and EGF/Gastrin Group .EGF and Gastrin were given to the rats in the EGF/Gastrin G roup （EG F 1μg .kg - 1 ，Gastrin 3μg ·kg - 1 ，once a day） for 14 days .At the end of the experiment ，Biochem ical items like fasting blood glucose ，serum insulin and C-peptide levels were tested while specimens were stained with HE method to observe the morphology changes .Results 　Fasting blood glucose levels of rats in the EGF/Gastrin Group were much lower than those in the DM Group （ P ＜ 0 .05） while the serum insulin ，C-peptide level and the pancreatic β cell amounts in the EGF/Gastrin Group were much higher or more than those in the DM Group（ P ＜ 0 .05） .Conclusion 　The combined use of EGF and Gastrin can lower the blood glucose of diabete rats and increase the content of serum insulin and C -peptide .However ，pancreatic β cell amounts were increased and their function were improved .