改良Giemsa法和硼酸美蓝法检测幽门螺杆菌感染的临床比较

摘要

Objective To investigate and compare modified Giemsa (M-G ) staining and boracic acid methylene blue (BAMB)staining on Helieobacter pylori detection .Methods We enrolled 80 patients diagnosing as chronic superficial gastritis and gastric ulcer,then we used Modified Giemsa (M-G) staining and boracic acid methylene blue (BAMB ) staining to evaluate HP infection of gastric mucosa and com pared the positive detection rate of the above two methods.Results The sensitivity ,specificity ,and concordance rate of M-G was 100％ ,96.2％ (50/52) and 97.5％ (78/80).Those of BAMB was 100％ ,88.5％ (46/52) and 92.5％ (74/80).The specificity of HP with M-G was significantly higher than that with BAMB (P＜0.05) .There were not significantly difference in the sensitivity and concordance rate of M-G and BAMB(P＞0.05).Conclusion M-G and BAMB are all the most sensitivity and differential method .But the specificity of HP with M-G is better than that with BAMB.%目的 探讨改良Giemsa法和硼酸美蓝法在检测胃幽门螺杆菌(Hp)感染中的应用价值.方法 收集80例慢性浅表性胃炎及胃溃疡患者,分别采用改良Giemsa法和硼酸美蓝法检测胃粘膜Hp,以Hp菌培养和C13呼气试验联合检测结果为Hp感染标准,比较两种检测方法对Hp检测的应用价值.结果 以Hp菌培养和C13呼气试验联合检测结果为对照,改良Giemsa法检测Hp感染的特异性为100%,敏感性为96.2%(50/52),诊断符合率为97.5%(78/80);硼酸美蓝法检测Hp感染的特异性为100%,敏感性为88.5%(46/52),诊断符合率为92.5%(74/80).两种方法检测Hp感染的特异性、敏感性及诊断符合率均较高,特异性和诊断符合率比较差异无显著意义(P＞0.05),但改良Giemsa法的检测敏感性明显高于硼酸美蓝法(P＜0.05).结论 改良Giemsa 染色法和硼酸美蓝法具有较高的Hp感染检测特异性和诊断符合率,均为比较理想的常规Hp检测染色法.