脓毒症大鼠肠道及腹水中菌群变化研究

摘要

目的 定量检测脓毒症大鼠肠道主要菌群的变化情况,同时找出引起脓毒症大鼠患病甚至死亡的主要致病菌.方法 采用盲肠结扎穿孔法(CLP)制作大鼠脓毒症模型,取腹水进行细菌培养和菌种鉴定;同时取结肠内容物提取DNA,使用荧光定量PCR法对肠道内5种细菌进行定量.结果 与对照组相比模型组大鼠肠道内双歧杆菌、乳杆菌数量明显减少,而大肠埃希菌的数量则有所增加.同时大肠埃希菌在腹水中大量繁殖.结论 肠道中益生菌数量减少和条件致病菌的大量繁殖是导致脓毒症大鼠发病和死亡的微生态学因素.%Objective To detect the m ain intesthalm icroflora and find the pathogen of rat septic model .M ethods The rat septicmodelwas induced by cecal ligation and puncture (CLP) .The variety of bacteria in asciteswas detem ined .In the same tine , the DNA that extracted Son oobnic contents was detected by fluorescent quantitative PCR for quantitating five kinds of bacteria .R e-suite The num ber of Bifidobacteria and Lactobacillus in model group was significantly decreased com pared with that of control group . The num ber of E scherichia coli in m odel group ,how ever ,w as significantly increased can pared w ith that of control group .o n the other hand ,there were considerable Escherichia coli in ascites .Conclusion The reduction of intestinal probiotical bacteria and the large population of conditioned pathogen are the cause of morbidity and mortality in septic rate form icroecological factors .