Objective To evaluate the significance in expression of protein pl6 which is the product of tumor-suppressing gene P16ink4a in liquid-based cytology to cervical screening and the confidence to be the biological marker of cervical intraepithelial neo-plasia(CIN) .Methods SP immunohistochemistry detection of protein pl6 was performed on 78 specimens remaining from liquid-based cytology which HPV detection was positive ,and compared with the results of biopsy histology .Results In patients with cytolog-ical diagnosis of negative for intraepithelial lesion or malignancy (NILM ) ,atypical squamous cells (ASC ) ,low-grade squamous intraep-ithelial lesion(LSIL) ,and high-grade squamous intraepithelial lesion (HSIL) ,the positive rates of protein pl6 were 0(0/20)、38.9％ (14/36),25 .0％ (5/2)、85 .0％ (17/20),respectively .The positive rate in ASC and HSIL groups was significantly higher than NILM group( ρ＜0 .05) .Whereas for LSIL group was not ( ρ＞0 .05) .Compared with the results of biopsy histology ,one case was positive of protein pl6 in chronic cervicitis group (2 .70％ ,1/36) ,40 cases in CIN (72 .7％ ,40 /55 ) ,and 5cases in SCC (100 .0％ ,5 / 5 ) .The difference in the positive rates between the first two groups ( ρ＜0 .05 ) .Conclusion The abnormal expression of protein pl6 has connected with the development of CIN ,which can become the maker of CIN to help cytology screening .%目的 探讨肿瘤抑制基因P16ink4a的编码产物p16蛋白的表达在宫颈液基薄层细胞学(liquid based cytological test,LCT)筛查中的意义及作为宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)生物标志物的可行性和临床意义.方法 选择96例HPV阳性的宫颈液基薄层细胞学剩余标本,制作液基薄片,利用SP免疫组化法检测LCT标本中p16蛋白,并与组织活检结果进行对照.结果 p16蛋白在宫颈细胞学诊断未见上皮内病变及癌变(NILM)、非典型鳞状上皮细胞(ASC)、低度鳞状上皮内病变(LSIL)、高度鳞状上皮内病变(HSIL)的阳性表达率分别为0(0/20)、38.9%(14/36),25.0%(5/20)、85.0%(17/20).其中ASC及HSIL组的阳性率显著高于NILM组(P＜0.05),而LSIL组与NILM组比较差异则无统计学意义(P＞0.05).与病理组织作对照,病检为慢性宫颈炎的病例中,有1例在LCT上p16蛋白表达阳性(2.70%,1/36);各级CIN中40例表达阳性(72.7%,40/55);5例SCC全部表达阳性(100.0%,5/5),且前两组阳性率差异有统计学意义(P＜0.05).结论 p16蛋白异常表达与CIN的发生有关,其可作为CIN的标志物,辅助细胞学筛查.
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