皮肤鳞癌组织中p14ARF、E-cad、FOS和JUN 基因甲基化状态的分析

摘要

目的 通过检测皮肤鳞癌及正常皮肤组织中p14ARF、E-cad、FOS、JUN基因启动子区域CpG岛的甲基化状态,探讨p14ARF、E-cad、FOS、JUN在皮肤鳞癌发生过程中的作用及诊断意义.方法 采用甲基化特异性PCR(MSP)技术,检测30例皮肤鳞癌组织和20例正常皮肤组织基因启动子区附近CpG岛的甲基化情况.结果 30例皮肤鳞癌组织中发现p14ARF、E-cad基因高甲基化例数分别为18例(60.0%)和11例(37.6%),而20例正常组织分别为1例(5.0%)和2例(10.0%);30例皮肤鳞癌FOS、JUN基因非甲基化分别为22例(73.3%)和25例(83.3%);20例正常组织非甲基化分别为6例(30.0%)和1例(5%).采用χ2检验进行统计分析,存在统计学差异(P<0.05).结论 p14ARF、E-cad、FOS、JUN基因启动子区域异常甲基化状态与皮肤鳞癌发病联系紧密.%Objective To investigate the abnormal promoter mcthyiation status of p17ARF、E-cad、FOS、JUN in skin squamous cell carcinoma. To analyze the value of these genes in the diagnosis of skin squamous cell carcinoma. Methods Promotor mcthylation of p14ARF、E-cad、FOS and JUN in 30 cases of skin squamous cell carcinoma,and 20 cases of normal skin tissues were detected by mcthylation-spccific PCR. Results The hypcrmcthylation rate of p14ARF、E-cad were 60. 0 % and 37. 6 % respectively in cancer specimen; while the mcthylation rate of p14、ARF、E-cad were 5. 0% and 10. 0% respectively in the normal tissuc;Thc hypomethylation rate of FOS、JUN were 73. 3% and 83. 3% respectively; while the hypomcthylation rate of FOS、JUN in normal group were just 30. 0% and 5. 0% respectively. Significant difference between mormal and tumor group. Conclusion The promotor mcthylation of p14ARF、E-cad、FOS、JUN was related closely to the progression of skin squamous cell carcinoma, the mcthylation status of p14ARF、E-cad、OS and JUN may be applied as prognosis markers in skin squamous cell carcinoma.