临床分离嗜麦芽窄食单胞菌的耐药基因检测及分子分型研究

摘要

目的 研究临床分离嗜麦芽窄食单胞菌的耐药基因分布及分子分型,为分子流行病学研究以及抗感染治疗提供依据.方法 采用PCR法对18株嗜麦芽窄食单胞菌进行L1、L2的基因筛查,利用多位点序列分析(MLST)技术进行分子分型研究.结果 18株嗜麦芽窄食单胞菌中L1基因13株,全部具有L2基因.MLST结果显示,18株临床菌株中ST31型6株;ST4型4株; ST25、ST29型各3株;ST8、ST28型各2株;发现两株新型.结论 耐药基因L1、L2存在于多数嗜麦芽窄食单胞菌中,可能是导致该菌对β内酰胺类耐药的主要原因.MLST提供了嗜麦芽窄食单胞菌分子流行病学研究的新方法.%Objective Analysing the drug-resisitant gene distribution and molecular typing of Stenotrophomonas marnltophilia which isolated from clinical in order to provide the evidence of molecular epidemiological study and anti-infecrntive therapy. Methods Using PCR for the 18 Stenotrophomonas maltophilia strains'gene screening of L1 and L2 ;makrning the molecular typing analysis by MLST. Results There are 13 strains of Stenotrophomonas mahophilia have L1rngene,all of them have L2 gene. MLST shows that 6 strains have the same ST number 31,4 strains are ST4,3 strainsrnare ST25 and ST29.2 strains are ST number 8 and ST28. Conclusion The drug resistance gene L1 and L2 consist in vast majority Stenotrophomonas mahophilia,it maybe the main reason of resistant to β-lactam; MLST provide the newrnmethod for researching the Molecular epidemiological study of Stenotrophomonas mahophilia.