肝螺杆菌诱导不同品系小鼠慢性肝损伤与 Th1／Th2细胞功能变化的分析

摘要

Objective To observe the relationship between liver pathological injury induced by H.hepaticus in mice of different strains and Th1/Th2 cell function.Methods SPF-class male BABL/c Cr、SCID/Cr,C57BL/6 Cr mice inoc-ulated H.hepaticus standard strain ATCC51450 0.2ml bacterial suspension (1×108 CUF/ml),for 3 times,each time in-terval of 48 hours,the control Group gavage the same amount of PBS.At last after Helicobacter hepaticus inoculation 16 weeks mice were killed,specimens from serum and liver,one liver tissue for histopathology;application of ELISA method to detect cytokines IFN-γ,TNF-α,IL-4,IL-5 contents of another liver tissue and serum.Results After H.he-paticus infection 16 weeks,IFN-γ,TNF-α contents of serum and liver tissue in BALB /c Cr mice were significantly higher than the control group(P <0.001);which IFN-γ,TNF-αcontents of serum and liver tissue in BALB/c Cr mice were significantly higher than SCID/Cr mice and C57BL/6 Cr mice (P <0.001);Compared with the control group,the IFN-γ,TNF-αcontents of serum and liver tissue in SCID/ Cr mice and C57BL/6 Cr mice after H.hepaticus infection were no significant difference (P >0.05);Compared with the control group,the IL-4,IL-5 contents of serum and liver tissue in all strains of mice after H.hepaticus infection were no significant difference (P >0.05);IFN-γ,TNF-α con-tents of serum and liver tissue in H.hepaticus infected BALB/c Cr mice were increased with liver pathology scores in-creased (P < 0.05;P < 0.01).Conclusion The degree of chronic liver injury induced by H.hepaticus infection in BALB/c Cr mice were positively correlated with IFN-γ,TNF-α levels of Th1 cytokines;Host immune response after immunocompetent mice infect H.hepaticus is a Th1-based immune response.%目的：观察不同品系小鼠感染 H．hepaticu 后的肝脏病理损伤与 Th1／Th2细胞功能的变化的关系。方法SPF 级雄性 BABL／c Cr、SCID／ Cr、C57BL／6 Cr 小鼠接种 H．hepaticus 标准菌株 ATCC51450菌液0．2 ml（1×108 CUF／ml），连续3次，每次间隔48 h，对照组灌饲等量的 PBS。于末次接种 H．hepaticus 后第16周处死小鼠，留取血清和肝脏，一份肝组织行病理组织学检查，另一份肝组织及血清应用 ELISA 方法检测细胞因子 IFN-γ、TNF-α、IL-4、IL-5含量。结果H．hepaticus 感染后16周时 BALB／c Cr 小鼠血清、肝脏组织中 IFN-γ、TNF-α含量明显高于对照组（P ＜0．001）；BALB／c Cr 小鼠血清、肝脏组织中 IFN-γ、TNF-α含量明显高于 SCID／ Cr、C57BL／6 Cr 小鼠（P ＜0.001）；而 SCID／ Cr 小鼠、C57BL／6 Cr 小鼠血清、肝脏组织中 IFN-γ、TNF-α含量与对照组相比无显著性差异（P ＞0.05）；H．hepaticus 感染后的各品系小鼠血清、肝脏组织中 IL-4及 IL-5含量与对照组相比，无显著性差异（P ＞0.05）；H．hepaticus 感染后 BALB／c Cr 小鼠血清及肝脏组织中 IFN-γ、TNF-α含量随着肝脏病理评分的增高而增加（P ＜0．05；P ＜0．01）。结论H．hepaticus 感染 BALB／c Cr 小鼠后诱导慢性肝损伤的程度与 Th1细胞因子 IFN-γ、TNF-α的水平呈正相关；具有免疫活性小鼠 H．hepaticus 感染后宿主免疫反应是以 Th1细胞为主的免疫反应。