Objective :To investigate the effects of astragalus polygonum compound on balance of renal endothelin ( ET - 1 )and nitric oxide ( NO ) in obesity rats.Methods : To establish experimental obese rat model through a high sugar high fat diet.Animals were administered with compound Astragalus Polygonum soup for 8 weeks.Then serum sample were used to detect TG、TC 、Scr、BUN and Glu.ET - 1 and NOS in renal cortical tissue were detected with RIA and colorimeter.Renal ET - 1 and NOS mRNA expressions were detected through reverse transcription - polymerase chain reaction.Results : The body weight of rats treated with compound Astragalus Polygonum was decreased significantly compared with model group, and there is similar results in rats treated with Benazepril ( P ＜ 0.05 ); serum LDL、Scr and BUN could significantly reduce through treated with compound Astragalus Polygonum ( P ＞ 0.05 ) .hut there were no significant effects on TC , TG 、HDL - C 、Glu and insulin ( P ＞ 0.05 ) ; with the normal group , the obese rat renal NOS activity and gene expression level decreased significantly, while the ET - 1 and ET - 1 mRNA expression increased significantly , by the Chinese intervention After treatment, upregulation of NOS and NOS mRNA.ET - 1 and ET - 1 mRNA was decreased.Conclusion :Compound Astragalus Polygonum could prevent the renal function of obesity rats,reduce body weight and blood lipid,it also participates in the maintenance of renal homeostasis NO/ET - 1.%目的:探讨肥胖大鼠肾组织内皮素(ET-1)和一氧化氮(NO)平衡及中药复方黄芪首乌汤对其干预作用.方法:采用高糖高脂饲料喂养建立实验性肥胖大鼠模型,以复方黄芪首乌汤灌胃治疗,8周末采血检测血脂、血糖和肾功能,并取肾皮质匀浆检测ET-1和NOS含量,逆转录-聚合酶链反应检测肾组织ET-1和NOS mRNA表达.结果:复方黄芪首乌汤组大鼠体重显著下降,与苯那普利对照组结果相似(P＜0.05);复方黄芪首乌汤能够明显降低肥胖大鼠血清LDL(P＞0.05),但对实验大鼠的TC、TG和HDL-C作用不显著(P＞0.05);复方黄芪首乌汤对实验大鼠血糖和Ins作用不明显(P＞0.05);复方黄芪首乌汤能够下调实验鼠血肌酐和尿素氮水平(P＜0.05);与正常组比较,肥胖大鼠肾组织NOS活力和基因表达水平显著下降,而ET-1及ET-1 mRNA表达水平显著上升,经中药干预治疗后NOS及NOS mRNA表达上调,ET-1及ET-1 mRNA表达下调.结论:复方黄芪首乌汤对大鼠肥胖具有一定的防治作用,可以减轻大鼠体重,调节血脂紊乱,保护肾脏功能,同时参与维持肾组织NO/ET-1动态平衡有关.
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