绞股蓝总皂苷对四氯化碳诱导大鼠肝纤维化的防治作用

摘要

目的:观察绞股蓝总皂苷对四氯化碳( CCl4)诱导的大鼠肝纤维化的防治作用.方法:采用CCl4诱导的大鼠肝纤维化模型,分为正常组(Z,n=6)、模型组(M,n=8)、绞股蓝总皂苷组(J,n=8)、秋水仙碱(Q,n=8).造模6周末开始给药(股蓝总皂苷200mg/kg体重、秋水仙碱0.1mg/kg体重),给药3周.观察:①大鼠体重、肝脾比值的变化；②血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、谷氨酰转肽酶(GGT)活性、白蛋白( Alb)、总胆红素(TBil)含量、肝组织羟脯氨酸(Hyp)含量；③肝组织超氧化物歧化酶(SOD)活性及丙二醛(MDA)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)含量；④肝组织病理及胶原沉积情况.结果:①M组大鼠血清ALT、AST、GGT、TBil显著升高,Alb显著降低；J和Q组大鼠血清ALT、AST、GGT、TBil显著下降,Alb显著升高；②M组大鼠肝组织Hyp含量显著升高,J组及Q组大鼠肝组织Hyp含量显著下降；③肝组织HE染色显示:M组大鼠肝细胞脂肪变性,大量纤维结缔组织增生,假小叶形成.J组及Q组大鼠肝细胞脂肪变性减轻,纤维增生减少,少见完整假小叶结构.天狼星红染色显示:M组大鼠肝窦周胶原沉积明显,形成较厚汇管区和中央静脉间的纤维间隔,J组和Q组大鼠肝脏汇管区胶原纤维染较M组明显减轻；④M组大鼠肝组织SOD活性及GSH含量明显降低,MDA含量显著升高.J组大鼠肝组织SOD活性显著提高.结论:绞股蓝总皂苷具有显著抗CCl4诱导的大鼠肝纤维化及氧化损伤的作用.%Objective: To investigate the effect of gypenoside on liver fibrosis induced by carbon tetrachloride ( CC1,) in rats. Methods; liver fibrosis was induced by CC14 injected subcutaneously twice per week for nine weeks and rats were divided into normal (Z, n = 6), model (M, n = 8 ) , gypenoside (J, n = 8) and colchicine (Q, n = 8) groups. Rats were administrated with gypenoside (200mg/kg body weight) and colchicine (0. lmg/kg body weight) respectively for three weeks. Serum samples were collected for alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyhransferase (G-GT), albumin (Alb) and total bilirubin (TBil) assay. Liver tissues were harvested for hydroxyproline (Hyp), superoxide dismutase (SOD), Glutathione (GSH) , glutathione peroxidase (GSH-Px) and maleic dialdehyde (MDA) detection. Histological changes and collagen deposition were observed in H. E. and Sirius red staining sections. Results: Serum ALT, AST, G-GT, TBil and hepatic Hyp increased and Alb decreased significantly in model group. After gypenoside and colchicine administration, serum ALT, AST, G-GT, TBil and hepatic Hyp were inhibited significantly, and especially, with gypenoside treatment, serum Alb increased significantly. Histological detetion showed that serious steatosis, inflammation and fibrosis as well as collagen depostion were observed in the liver in model group and these pathological changes were alleviated by gypenoside and colchicine. On the other hand, hepatic SOD, GSH decreased and MDA increased in model group. Gypenoside increased hepatic SOD and colchicine improved hepatic CSH remarkably. Conclusion: Gypenoside can inhibit liver fibrosis induced by CC14 in rats obviously.