黄根单体对大鼠肝星状细胞增殖和抑制作用及诱导其凋亡的影响

摘要

Objective:To investigate the effects of Prismatomeris connata Y.Z.Ruan.Monomers on Rat Hepatic Stellate Cell (HSCT6).Methods:MTT assay was used to evaluate the inhibitory effect of the drugs on HSC-T6,flow cytometry were applied to measure the cell apoptosis.Results:HG3 not only could inhibit proliferation of HSC-T6 cells in concentration manners,but also could induce apoptosis of HSC-T6 cells.HG2 and HG4 showed a slight effect on the Proliferation apoptosis in Rat Hepatic Stellate Cell.Conclusion:The Prismatomeris connata Y.Z.Ruan.Monomers can inhibit the proliferation of HSC-T6 cells and induce apoptosis,wihch show the therapeutical effects on hepatic fibrosis.%目的:研究黄根单体(HG2、HG3、HG4)对大鼠肝星状细胞(HSC-T6)增殖的抑制作用以及诱导其凋亡的影响.方法:采用四甲噻唑蓝(MTr)法检测黄根单体对HSC-T6的增殖的抑制作用,并运用流式细胞术检测细胞凋亡率.结果:单体HG3可诱导HSC-6发生凋亡,对其增殖具有抑制作用,且具有剂量依赖性;而单体HG2和单体HG4对大鼠HSC-6表现出较弱的抑制和诱导凋亡作用.结论:黄根的蒽醌类成分能抑制HSC-T6细胞增殖并诱导其发生凋亡,从而发挥抗肝纤维化作用.