参麦注射液对实验性心力衰竭大鼠左室舒缩性能rn及血浆AngⅡ、ET和ANP的影响

摘要

Objective:To investigate the effects of Shenmai injection (SMI,参麦注射液) on the plasma angiotensin(AngⅡ),endothelin(ET),atrial natriuretic polypeptide(ANP) and the myocardial contractility and relaxation of left ventricular in experimental rats with heart failure(HF).Methods:The wistar rat model with HF was produced by constricting abdominal aorta.Hemodynamic parameters,including maximum rate of intraventricular pressure rise(+dp/dtmax),left ventricular systolic pressure(LVSP),maximum velocity of contractile element shortening(Vmax),maximum rate of intraventricular pressure down(dp/dtmax) and left ventricular end diastolic pressure(LVEDP) were measured by the method of catherization.Plasma concentrations of ANP,ET and AngⅡ were determined by immunoradiometric assay.Results:In high dose SMI group,the LVSP,+dp/dtmax,dp/dtmax and Vmax were increased by 17.31%,10.60%,18.76% and 23.24%;LVEDP was decreased by 56.15%,plasma concentrations of AngⅡ,ET and ANP were decreased by 32.65%,38.52% and 9.44%,respectively.In comparison with those in model group,the differences were significant (all P<0.05).In middle dose group,the LVSP,+dp/dtmax,dp/dtmax and Vmax were increased by 14.76%,2.97%,14.19% and 11.40%,AngⅡ,ET and ANP were decreased by 22.47%,34.65% and 17.18%,respectively,the differences were significant in comparison with those in model group (all P<0.05).In low dose SMI group,the indices of hemodynamics were not considerable difference except LVEDP,but levels of plasma ANP,ET and AngⅡ were significantly lower than those in model group,decreased by 18.95%,33.59% and 16.77%,respectively.Conclusions:SMI can reduce the plasma concentrations of AngⅡ,ET and ANP,and improve the myocardial contractility and relaxation of left ventricular in heart failure rats.%目的：研究参麦注射液对实验性心力衰竭(心衰)大鼠左室舒缩功能及血浆血管紧张素Ⅱ(AngⅡ)、内皮素(ET)和心钠素(ANP)的影响。方法：采用缩窄腹主动脉方法复制大鼠心衰模型。左心室插管术测定血液动力学指标；放免法测定血浆AngⅡ、ET和ANPⅡ浓度。结果：心衰大鼠左室舒缩功能减退，血浆AngⅡ、ET和ANP水平显著升高；与假手术组相比，模型组大鼠血浆AngⅡ、ET、ANP浓度分别升高56.21%、37.83%和44.42%。与模型组相比，参麦注射液高剂量组大鼠Vmax、+dp/dtmax 、dp/dtmax、左心室内压峰值(LVSP)分别升高23.24%、10.60%、18.76%和17.31%，左心室舒张末压(LVEDP)降低56.15%，血浆AngⅡ、ET、ANP浓度分别降低32.65%、38.52%和9.44%，均有显著性差异(P均<0.05)；参麦注射液中、低剂量组大鼠各项指标均有不同程度的改善。结论：参麦注射液能改善心衰大鼠左室舒缩功能，降低血浆AngⅡ、ET浓度，影响 ANP分泌。