电针对老年痴呆大鼠海马 N-甲基-D-天冬氨酸受体1mRNA的影响

摘要

目的 观察电针对老年痴呆(AD)模型大鼠海马N-甲基-D-天冬氨酸受体1(NMDAR1)mRNA的影响,探讨其作用机制.方法 将SD大鼠按随机数字表分为4组,即正常组、假手术组、模型组、电针组,每组10只.采用双侧海马一次性注射聚集态Aβ25-35制备AD大鼠模型,电针组选取双侧"肾俞"、"内关"和"大椎",接G6805-Ⅱ型电针治疗仪,选用2 Hz连续波,强度为1 mA,通电20 min,每日1次,每周6次,共治疗2周.采用原位杂交法检测各组大鼠海马NMDAR1 mRNA的变化,并分析电针对其的影响.结果 模型组大鼠海马CA1、CA3区NMDAR1 mRNA阳性细胞平均灰度值与假手术组相比显著升高(P＜0.01).电针组大鼠海马CA1、CA3区NMDAR1 mRNA阳性细胞平均灰度值与模型组相比明显降低(P＜0.01).结论 电针可能通过上调NMDAR1 mRNA的表达,充分活化NMDAR,加强长时程增强效应,从而改善AD大鼠的学习记忆能力.%Objective To observe the effect of electro-acupuncture on the expression of NMDAR1 mRNA in the hippocampus of rats with Alzheimer's disease (AD), and discuss the mechanism. Methods SD rats were randomly divided into 4 groups：the normal group, sham-operated group, model group, electro-acupuncture group. Bilateral hippocampus were injected condensed Aβ25-35 to make rat model of AD. Electro-acupuncture group were selected bilateral Shenshu points, bilateral Neiguan points and Dazhui points, treated by G6805-Ⅱ type electro-acupuncture therapeutic apparatus with the frequence of 2 Hz continuous wave and the intensity of 1 mA, lasting 20 min, one time per day, 6 times a week, for a total of two weeks. Hybridization in situ was used to detect NMDAR1 mRNA changes in hippocampus of each group and the effect of electro-acupuncture's intervention to NMDAR1 mRNA was analyzed. Results Compared with sham-operated group, NMDAR1 mRNA's expression of model group's hippocampus decreased obviously (P＜0.01). Compared with the model group, NMDAR1 mRNA's expression of electro-acupuncture group's hippocampus increased significantly (P＜0.01). Conclusion Electro- acupuncture may increase the expression of NMDAR1 mRNA, make full activation of NMDAR, enhance LTP, to improve the learning-memory capacity of rats with AD.