细胞膜色谱法与液相色谱-质谱联用筛选佛手的活性成分

摘要

Objective To screen the active component from Fructus Citri Sarcodactylis which have aortic vascular relaxant effect. Methods The active components from Fructus Citri Sarcodactylis were screened by combining vascular smooth muscle/cell membrane chromatography (VSM/CMC) and liquid chromatography/mass spectrometry (LC/MS) with pharmacological assay in vitro. Results Bergapten was the active component of Fructus Citri Sarcodactylis that functions in vascular. The pharmacological experiment showed that bergapten had vasodilating effect. The capacity factor of this compound on the VSM/CMC model was found to be significantly correlated with its pharmacological effect. Conclusion Association between compound and membrane protein (receptor) can be reflected by using the VSM/CMC model. VSM/CMC-offline-LC/MS technology can be used to quickly screening new active components from natural medicine.%目的从佛手中筛选对主动脉血管有舒张作用的有效成分。方法采用大鼠血管平滑肌细胞膜色谱模型(VSM/CMC)筛选，液相色谱-质谱(LC/MS)离线分析，并结合离体药理实验确定佛手舒张血管的有效成分。结果佛手柑内酯是佛手中作用于血管的活性成分，药理实验结果表明其具有舒张血管作用，化合物在VSM/CMC的保留特性和其药理作用有显著相关性。结论 VSM/CMC 模型可以反映化合物与细胞膜及膜蛋白(包括受体)的相互作用；VSM/CMC 和LC/MS技术联用，为从天然产物中快速寻找新的活性成分提供了途径。