连翘药材及其配方颗粒中连翘酯苷A和连翘苷含量比较

摘要

目的：比较连翘药材及其配方颗粒中连翘酯苷A和连翘苷的含量。方法采用高效液相色谱梯度洗脱法。色谱柱：SunFire C18(4.6 mm×250 mm,5µ m)；流动相A为乙腈,流动相B为0.4%冰醋酸溶液；流速：1.0 mL/min；检测波长：277 nm；柱温：30℃。对连翘药材及其配方颗粒中连翘酯苷A和连翘苷的含量进行测定,比较二者含量的差异。结果连翘配方颗粒中连翘酯苷A的含量低于原药材中的含量,与所标示的浓缩倍数不符；配方颗粒中连翘苷的含量与原药材一致。结论原配方颗粒的生产工艺需要改进,配方颗粒需要建立完善的质量控制方法和合理的质量标准。%Objective To compare the contents of forsythoside A and forsythin in Fructus Forsythia and its dispensing granule. Methods HPLC-gradient elution method was used with SunFire C18 column (4.6 mm×250 mm, 5μm), mobile phase A of acetonitrile and B of acetic acid, flow rate of 1.0 mL/min, detection wavelength of 277 nm, and column temperature at 30 ℃. HPLC was used to determine the contents of forsythoside A and forsythin in Fructus Forsythia and its dispensing granule, and compare the difference between the two contents. Results The content of forsythoside A in dispensing granule was less than that of raw material of Fructus Forsythia, and the concentration of the major components in the commercial Lianqiao Granule were not equivalent to that in the decoction of Fructus Forsythia. The content of forsythin in dispensing granule was equivalent with that of raw material of Fructus Forsythia. Conclusion The original formula granule production process needs to be improved, and the standardized criteria for the quality control and reasonable quality standard of granule should be established.