HPLC测定肝泰舒胶囊中獐牙菜苦苷含量

摘要

目的：建立高效液相色谱方法测定肝泰舒胶囊中獐牙菜苦苷含量的方法。方法采用 Waters Symmetry-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.02%磷酸为流动相进行梯度洗脱,流速为1.0 mL/min,检测波长为250 nm,柱温为室温。结果獐牙菜苦苷在0.373556～2.988448μg范围内呈良好的线性关系(r2＝1.0000),平均回收率为106.81%,RSD＝3.7%。结论该方法快捷、准确、简便、重复性好,可用于肝泰舒胶囊的质量控制。%Objective To establish an HPLC method for determining the content of swertiamarin in Gantaishu capsules. Methods Using a Waters Symmetry C18 column (4.6 mm×250 mm, 5μm), the mobile phase was acetonitrile-0.02%phosphoric acid, with flow rate of 1.0 mL/min. The detection wavelength was at 250 nm, the column temperature was room temperature. Results The content of swertiamarin showed a good linear relationship in the range of 0.373 556-2.988 448 μg, r 2=1.000 0. The average recovery was 106.81%, and RSD was 3.7%. Conclusion This method is fast, accurate and simple with good reproducibility, which can be applied to the quality control of Gantaishu capsules.