银杏叶片剂大鼠体内多成分序贯代谢研究

摘要

目的：以序贯代谢思想为指导,研究银杏叶片剂成分体内代谢动态情况。方法以在体大鼠封闭肠环法为研究手段,结合色谱指纹图谱,对银杏叶片剂经过口服消化系统空间顺序代谢,即肠道菌群、肠壁细胞及肝代谢后的入血成分谱进行考察及对比研究。结果对可检测到的色谱峰分析显示,银杏叶片剂有12个色谱峰在肠道内被肠道菌群代谢,经肠壁代谢产生7个色谱峰,银杏叶片剂中的成分大部分被肝代谢,3个原药色谱峰以原型入血。结论本研究对银杏叶片剂在口服途径不同代谢部位的情况进行了定性描述,可为该制剂的质量控制、机制阐述及再次开发提供参考。%Objective To investigate dynamic metabolism in vivo of Ginkgo Folium Tablet under the guidance of sequential metabolism thoughts. Methods In situ closed-loop in rats was carried out to study sequential metabolism of Ginkgo Folium Tablet through oral digestive system, namely to investigate and compare the intestinal flora metabolism, the gut wall metabolism and hepatic metabolism, combined with chromatographic fingerprint of blood samples. Results The analysis showed that 12 peaks in Ginkgo Folium Tablet were metabolized by intestinal flora, and 7 peaks generated through the gut wall. Most components of Ginkgo Folium Tablet were metabolized in liver, and 3 original medicine components were directly into the blood. Conclusion This study conducts a qualitative description of metabolism of Ginkgo Folium Tablet in different parts of the oral route, and provides references for the quality control, mechanism explanation and secondary development for Ginkgo Folium Tablet.